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Alexa Fluor® 594 anti-MECP2 Antibody

Pricing & Availability
Clone
N227/21 (See other available formats)
Regulatory Status
RUO
Other Names
Methyl-CpG-binding protein 2, meCp-2 protein, RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N227slash21_A594_MECP2_Antibody_IHC-P_1_080118
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N227slash21_A594_MECP2_Antibody_IHC-P_1_080118
    IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N227slash21_A594_MECP2_Antibody_IHC-P_2_080118
    IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N227slash21_A594_MECP2_Antibody_IHC-P_3_080118
    IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse thalamus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N227slash21_A594_MECP2_Antibody_IHC-P_4_080118
    IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability Save
814005 25 µg 104€
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814006 100 µg 169€
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Description

Methyl CpG binding protein (MECP2), also known as autism susceptibility to X-linked 2 (AUTSX3), is a nuclear protein crucial for normal neuronal function that binds to methylated DNA, forming complexes with histone deacteylases and turning off gene expression.  MECP2 expression is an X-linked trait. Loss of MECP2 expression causes Rett Syndrome (also known as RTS, RTT, or RS) a neurological disorder which causes decelerated growth of hands, feet, and the head.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 157-169 (FTVTG[dimethyl-R]GSPSRRE) of human MECP2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2750412 (BioLegend Cat. No. 814005)
AB_2750412 (BioLegend Cat. No. 814006)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Gene ID
4204 View all products for this Gene ID
UniProt
View information about MECP2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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