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Alexa Fluor® 647 anti-mouse Aiolos Antibody

Pricing & Availability
Clone
8B2 (See other available formats)
Regulatory Status
RUO
Other Names
IKAROS family zinc finger 3 (Ikzf3), zinc finger protein Aiolos, Zfpn1a3, Znfn1a3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8B2_AF647_Aiolos_Antibody_FC_1_022014
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488, fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with Aiolos (clone 8B2) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 8B2_AF647_Aiolos_Antibody_FC_1_022014
    C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488, fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with Aiolos (clone 8B2) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 8B2_AF647_Aiolos_Antibody_FC_2_022014
Compare all formats See Alexa Fluor® 647 spectral data
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653205 25 µg 128€
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653206 100 µg 315€
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Description

Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70% homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator.  Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal half of mouse Aiolos fused to GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, immunoprecipitation1, and Western blotting2.

Does not cross-react with human.

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes.  Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited.  In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (ICFC, IP)
  2. Thompson EC, et al. 2007. Immunity 26:335. (WB)
Product Citations
  1. Tauber M, et al. 2023. J Exp Med. :220. PubMed
RRID
AB_2563238 (BioLegend Cat. No. 653205)
AB_2563238 (BioLegend Cat. No. 653206)

Antigen Details

Structure
509 amino acids with predicted MW of 58 kD
Distribution

Nucleus, cytoplasm
Restricted to lymphoid lineage, expressed in B, NK and T cells

Function
Transcription factor that plays an important role in the regulation of lymphocyte differentiation; plays an essential role in regulation of B-cell differentiation, proliferation, and maturation to an effector state
Interaction
Homodimer and heterodimer with other IKAROS family members; interacts with IKZF4, IKZF5, IKZF1, HRAS, FOXP3, and BCL21L isoform Bcl-X(L); associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Morgan B, et al. 1997. EMBO J. 16:2004.
2. Wang JH, et al. 1998. Immunity 9:543.
3. Cariappa A, et al. 2001. Immunity 14:603.

Gene ID
22780 View all products for this Gene ID
UniProt
View information about Aiolos on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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