Alexa Fluor 647 anti-mouse TCR Vbeta11 Antibody anti-TCR V beta 11

Pricing & Availability

Clone: KT11 (See other available formats)
Regulatory Status: RUO
Other Names: T cell receptor Vβ 11 [vb11]
Isotype: Rat IgG2b, κ
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Product Citations: publications

B57BL/6 splenocytes stained with CD3 (145-2C11) PE and KT11 Alexa Fluor® 647

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125909

100 µg

212€

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Description

The Vβ11 gene locus is deleted in mice of TCR a haplotype (C57BR, C57L, SJL, SWR) and TCR c haplotype (RIII).Mice of TCR b haplotype (C57BL, BALB/c, AKR, etc) have Vβ11-bearing T cells .However, mice expressing MHC class II I-E and some superantigens, such as Mtv-8, Mtv-9 and Mtv-11 will show a partial deletion of Tb11-bearing T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_1227774 (BioLegend Cat. No. 125909)

Antigen Details

Distribution: Positive on a subset of alpha/beta T cells
Ligand/Receptor: MHC/peptide
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Gene ID: 21577 View all products for this Gene ID
UniProt: View information about TCR Vbeta11 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All TCR Vβ11 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse TCR Vβ11	KT11	FC
PE anti-mouse TCR Vβ11	KT11	FC
Alexa Fluor® 647 anti-mouse TCR Vβ11	KT11	FC
PerCP/Cyanine5.5 anti-mouse TCR Vβ11	KT11	FC
PE/Cyanine7 anti-mouse TCR Vβ11	KT11	FC
APC anti-mouse TCR Vβ11	KT11	FC
APC/Cyanine7 anti-mouse TCR Vβ11	KT11	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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