- Clone
- AE-1/AE-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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914211 | 25 µg | 148€ |
AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Reported Reactivity
- Dog, Non-Human Primate
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using human epidermal keratins.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For use of this antibody (clone AE-1/AE-3) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation/permeabilization with 100% ice-cold methanol alone.
For use of this antibody (clone AE-1/AE-3) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using Cyto-FastTM Fix/Perm Buffer Set (Cat. No. 426803).
For use of this antibody (clone AE-1/AE-3) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703).
Antigen Details
- Distribution
-
Cytoplasm, cell membrane
- Function
- Cytoskeleton, cell motility and structure
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
- Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105.
- Jiang S, et al. 2022. Immunity. 55:1118.
- Tiago M, et al. 2020. Br J Cancer. 122:789.
- Kou R, et al. 2018. Oncol Rep. 7:2013.
- Xie S, et al. 2020. Ann Transl Med. 1.384027778.
- Chang TH, et al. 2020. Cell Death Dis. 11:338.
- Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262.
- Cai S, et al. 2022. iScience. 25:104980.
- Gene ID
- NA
- UniProt
- View information about Pan-Cytokeratin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Pan-Cytokeratin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Pan-Cytokeratin | AE-1/AE-3 | IHC-P |
Alexa Fluor® 594 anti-Pan-Cytokeratin | AE-1/AE-3 | IHC-P,ICC,ICFC |
TotalSeq™-Bn1301 anti-Pan-Cytokeratin | AE-1/AE-3 | SB |
PerCP/Cyanine5.5 anti-Pan-Cytokeratin | AE-1/AE-3 | ICFC,SB |
Alexa Fluor® 647 anti-Pan-Cytokeratin Antibody | AE-1/AE-3 | IHC-P,ICC,ICFC |
Alexa Fluor® 488 anti-Pan-Cytokeratin | AE-1/AE-3 | IHC-P,ICC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Pan-Cytokeratin
IHC staining of purified anti-Pan-Cytokeratin antibody (clon... Formalin-fixed paraffin-embedded human tonsil treated with a... -
Alexa Fluor® 594 anti-Pan-Cytokeratin
Formalin-fixed paraffin-embedded human tonsil tissue stained... MCF7 cells were fixed with 4% paraformaldehyde, permeabilize... MCF7 cells (positive control) (closed histogram), and Jurkat... -
TotalSeq™-Bn1301 anti-Pan-Cytokeratin
-
PerCP/Cyanine5.5 anti-Pan-Cytokeratin
MCF-7 cells (positive cell line, solid histogram) were fixed... Jurkat cells (negative cell line, solid histogram) were fixe... Multiplexed IHC staining of PerCP/Cyanine5.5 anti-Pan-Cytoke... -
Alexa Fluor® 647 anti-Pan-Cytokeratin Antibody
MCF7 cells were fixed and permeabilized with 100% ice-cold m... IHC staining of Alexa Fluor® 647 anti-Pan Cytokeratin (clone... MCF-7 cells (positive cell line, filled histogram) and Jurka... -
Alexa Fluor® 488 anti-Pan-Cytokeratin
MCF7 cells were fixed and permeabilized with 100% ice-cold m... IHC staining of Alexa Fluor® 488 anti-Pan Cytokeratin (clone... MCF-7 cells (positive cell line, filled histogram) were fixe... Jurkat cells (negative cell line, filled histogram) were fix...
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