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Alexa Fluor® 647 anti-RNA Polymerase II RPB1 Antibody

Pricing & Availability
Clone
8WG16 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase II sub-unit 1, RPB1, CTD repeat YSPTSPS, C- terminal domain repeat YSPTSPS, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
8WG16_A647_RNA_Polymerase_II_RBB1_Antibody_012318
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 647 Mouse IgG2a (Cat. No. 400234)or with Alexa Fluor® 647 anti-RNA Polymerase II RPB1 (red) at (B) 1: 250 (2 µg/ml) and (C) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). Actin filaments were labeled with Flash Phalloidin™ Green 488 (green, Cat. No. 424201). The image was captured with a 60X objective.
  • 8WG16_A647_RNA_Polymerase_II_RBB1_Antibody_012318
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 647 Mouse IgG2a (Cat. No. 400234)or with Alexa Fluor® 647 anti-RNA Polymerase II RPB1 (red) at (B) 1: 250 (2 µg/ml) and (C) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). Actin filaments were labeled with Flash Phalloidin™ Green 488 (green, Cat. No. 424201). The image was captured with a 60X objective.
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664913 25 µg 128€
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664914 100 µg 324€
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Description

RPB1 is the catalytic and largest component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with RPB2, the second largest subunit. Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relatively to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single DNA template strand of the promoter is positioned within the central active site cleft of Pol II. Then, a bridging helix emanates from RPB1 and crosses the cleft near the catalytic site, which acts as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations during each neuocleotide addition. This promotes translocation of Pol II. Pol II moves on the template during transcription elongation. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembling factors that regulate transcription initiation, elongation, termination, and mRNA processing. It can act as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, being able to conform as both a replicate and transcriptase for the viral RNA circular genome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Wheat germ RNA Polymerase II
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.

Application References
  1. Edwards AM, et al. 1990. Proc. Natl. Acad. Sci. USA. 87:2122.
  2. Thompson NE, et al. 1990. J. Biol. Chem. 265:7069.
  3. Thompson NE, et al. 1989. J. Biol. Chem. 264:11511.
RRID
AB_2728497 (BioLegend Cat. No. 664913)
AB_2728497 (BioLegend Cat. No. 664914)

Antigen Details

Structure
RPB1 is a 220 kD subunit of RNA polymerase II and has 1970 amino acids.
Distribution

Nucleus; ubiquitous expressed in all cells

Function
Core element of RNA polymerase II transcription machinery.
Interaction
COMPASS (COMplex of Proteins ASsociated with Set1), SET2, BRE1
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Chávez S, et al. 2016. CUrr. Genet. 62:701.
Gene ID
5430 View all products for this Gene ID 20020 View all products for this Gene ID 2540292 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB1 on UniProt.org
Go To Top Version: 3    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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