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Alexa Fluor® 647 anti-SOX17 Recombinant Antibody (For Flow Cytometry)

Pricing & Availability
Clone
O92B6.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor SOX17, Transcription factor SOX 17, Transcription factor SOX-17, VUR3, SRY-Box17, SRY-Related HMG-Box Transcription Factor 17
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O92B6Rec_A647_ICFC_092624
OVCAR-3 cells (positive control, filled histogram), and HeLa cells (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-SOX17 Recombinant (clone O92B6.Rec) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400136) (open histogram, dashed line; representative histogram for both cell lines).
  • O92B6Rec_A647_ICFC_092624
    OVCAR-3 cells (positive control, filled histogram), and HeLa cells (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-SOX17 Recombinant (clone O92B6.Rec) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400136) (open histogram, dashed line; representative histogram for both cell lines).
Compare all formats See Alexa Fluor® 647 spectral data
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628157 25 tests 140€
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628158 100 tests 336€
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Description

SOX17 is an important member of the SOX family of transcription factors, which are defined by possession of a homologous sequence called the HMG (high mobility group) box. SOX genes are primarily involved in developmental processes including germ layer formation, cell-type specification, and organogenesis. SOX17 activates endodermal target genes, and is required for the formation of endoderm and blood vessel endothelium. SOX17 also plays critical roles in the maintenance of fetal and neonatal hematopoietic stem cells. Additionally, SOX17 is a regulator of human primordial germ cell fate.

SOX17 also acts as a tumor suppressor through suppression of Wnt signaling, and a reduction of SOX17 expression is found in gastrointestinal and colon carcinomas. Additionally, mutations in SOX17 can induce stem-like characteristics in tumor cells by causing SOX17 to dimerize with OCT4 and induce pluripotency transcriptional programs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant protein expressed in E. Coli corresponding to residues 177-414 of human SOX17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody is recommended for intracellular staining for flow cytometry (ICFC) but not immunocytochemistry (ICC) or immunohistochemistry (IHC use). This product has been optimized for flow cytometry application.

For use in ICC, and IHC on FFPE tissues (IHC-P), we recommend Cat. No. 628159 and 628160. This product has been optimized for imaging applications.

Antigen Details

Structure
SOX17 is a 414 amino acid protein with a predicted molecular weight of 44.1 kD.
Distribution

Nucleus

Function
Transcription factor
Interaction
Interacts with CTNNB1, LEF1, TCF4
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References
  1. Wegner M. 1999. Nucleic Acids Res. 27:1409-20.
  2. Du YC, et al. 2009. Gastroenterology. 137:1346-57.
  3. Zorn AM, et al. 1999. Mol Cell. 4:487-98.
  4. Sinner D, et al. 2004. Development. 131:3069-80.
  5. Hudson C, et al. 1997. Cell. 91:397-405.
  6. Matsui T, et al. 2006. J Cell Sci. 119:3513-26.
  7. Kim I, et al. 2007. Cell. 130:470-83.
  8. Zhang W, et al. 2008. Cancer Res. 68:2764-72.
  9. Tan DS, et al. 2020. Semin Cancer Biol. 67:65-73.
  10. Irie N, et al. 2015. Cell. 160:253-68.
Gene ID
64321 View all products for this Gene ID
UniProt
View information about SOX17 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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