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Alexa Fluor® 647 anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
4B10_AF647_T-Bet_Antibody_ICFC_1_021715
Human peripheral blood lymphocytes were surface stained with CD3 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 4B10_AF647_T-Bet_Antibody_ICFC_1_021715
    Human peripheral blood lymphocytes were surface stained with CD3 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 4B10_AF647_T-Bet_Antibody_ICFC_2_021715
Compare all formats See Alexa Fluor® 647 spectral data
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644803 25 µg 141€
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644804 100 µg 306€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  2. Zutshi S, et al. 2021. Immunology. 163:460. PubMed
  3. Mukherjee A, et al. 2021. Immunology. 164:318. PubMed
  4. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  5. Wang D, et al. 2018. Immunity. 48:659. PubMed
  6. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  7. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  8. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  9. Soderquest K, et al. 2017. PLoS Genet. 13(2):e1006587. PubMed
  10. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  11. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  12. Camell CD, et al. 2020. Cell Metabolism. 30(6):1024-1039.e6.. PubMed
  13. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  14. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  15. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  16. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
  17. Doss PMIA, et al. 2021. Cell Reports. 34(10):108833. PubMed
  18. Wieten R, et al. 2016. PLoS One. 11: 0149871. PubMed
  19. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  20. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  21. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  22. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
RRID
AB_1595573 (BioLegend Cat. No. 644803)
AB_1595573 (BioLegend Cat. No. 644804)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02/17/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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