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APC anti-human IL-9 Antibody

Pricing & Availability
Clone
MH9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
MH9A4_APC_IL-9_Antibody_090619
PMA and ionomycin-stimulated (6 hours, in the presence of monensin) Th2-polarized human lymphocytes were surface stained with CD3 FITC. Cells were fixed, permeabilized and intracellularly stained with IL-9 (clone MH9A4) APC (left), or mouse IgG2b, κ APC isotype control (right).
  • MH9A4_APC_IL-9_Antibody_090619
    PMA and ionomycin-stimulated (6 hours, in the presence of monensin) Th2-polarized human lymphocytes were surface stained with CD3 FITC. Cells were fixed, permeabilized and intracellularly stained with IL-9 (clone MH9A4) APC (left), or mouse IgG2b, κ APC isotype control (right).
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507613 25 tests 110€
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507614 100 tests 347€
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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Baculovirus-expressed, recombinant human IL-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity.

Application References
  1. Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528.
  2. Faulkner H, et al. 2002. J. Infec. Diseas. 185:665.
  3. Chen J, et al. 2008. Blood 111:5163. PubMed
  4. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ELISA) PubMed
  5. Lozano E, et al. 2012. J Immunol. 188:3869. PubMed.
Product Citations
  1. Lin T, et al. 2020. Viruses. :12. PubMed
RRID
AB_2814435 (BioLegend Cat. No. 507613)
AB_2814435 (BioLegend Cat. No. 507614)

Antigen Details

Structure
Cytokine; 32-39 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
3578 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org
Go To Top Version: 1    Revision Date: 09/06/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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