APC/Cyanine7 Streptavidin

Product Details

Verified Reactivity

Human, Mouse, Rat, All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

Streptavidin is conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The Streptavidin APC/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin APC/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Cyanine7 conjugation. For immunofluorescent staining, the recommended use of this reagent is =0.06 µg per 10⁶ cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Streptavidin-Allophycocyanin/Cyanine7 (APC/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Cyanine7 is 350 kD and Streptavidin alone is 52 kD.

Application References

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Antigen Details

Gene ID

NA

UniProt

View information about Biotin on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol
• Intracellular Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Certificate of Analysis