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Biotin anti-human HGF Antibody

Pricing & Availability
Clone
W19371A (See other available formats)
Regulatory Status
RUO
Other Names
Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19371A_BIOTIN_-HGF_Antibody_050323
Direct ELISA showing biotin anti-human HGF (clone W19371A) bound to plate-immobilized recombinant human HGF protein (Cat. No. 596402). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19371A_BIOTIN_-HGF_Antibody_050323
    Direct ELISA showing biotin anti-human HGF (clone W19371A) bound to plate-immobilized recombinant human HGF protein (Cat. No. 596402). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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613153 50 µg 188€
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Description

HGF is a dimeric molecule composed of the α-subunit and β-subunit, linked by a disulfide bond. The C-terminus of the α-subunit is followed directly by the N-terminus of the β-subunit. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases. This protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules, such as Bcl-xL. In addition, HGF prohibits Fas-mediated apoptosis signals via sequestration of Fas and Met receptor on cell surfaces. HGF also elicits the regression of fibrosis in numerous organs, such as scleroderma, cardiomyopathy, vocal scarring, and peritoneal fibrosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Recombinant HGF Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19371A) could function as a detection antibody when paired with purified anti-human HGF antibody (Cat. No. 613052) (clone W19372J) as the capture antibody and using Recombinant Human HGF protein (Cat. Nos. 596401 and 596402) as the assay standard.

RRID
AB_3097164 (BioLegend Cat. No. 613153)

Antigen Details

Structure
Heterodimer (alpha and beta chains)
Distribution

Mesenchymal cells, liver sinusoidal endothelial, macrophages

Function
Stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. TGFβ and angiotensin-II also depress HGF production in vitro.
Interaction
Hepathocytes, epithelial cells
Ligand/Receptor
MET receptor
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Kataoka H, et al. 2000. J Biol Chem. 275:40453-62.
  2. Wang X, et al. 2002. Mol Cell. 9:411-21.
  3. Wu MH, et al. 2004. Gene Ther. 11:170-80.
  4. Mizuno S, et al. 2008. Front Biosci. 13:7072-86.
  5. D'Angelo F, et al. 2013. Clin Exp Immunol. 174:60-72. 
Gene ID
3082 View all products for this Gene ID
UniProt
View information about HGF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All HGF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human HGF W19371A Direct ELISA
Biotin anti-human HGF W19371A Direct ELISA
Go To Top Version: 1    Revision Date: 05/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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