- Clone
- 100 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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658709 | 25 tests | 172€ |
Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application References
- Product Citations
-
- RRID
-
AB_2563283 (BioLegend Cat. No. 658709)
Antigen Details
- Structure
- Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
- Distribution
-
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
- Function
- Regulates apoptosis through controlling mitochondrial fusion and fission.
- Interaction
- BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852. - Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Bcl-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Bcl-2 | 100 | WB,IHC-F,IHC-P |
Alexa Fluor® 647 anti-Bcl-2 | 100 | ICFC,SB |
Alexa Fluor® 488 anti-Bcl-2 | 100 | ICFC |
PE anti-Bcl-2 | 100 | ICFC |
Brilliant Violet 421™ anti-Bcl-2 | 100 | ICFC |
TotalSeq™-Bn1180 anti-Bcl-2 | 100 | SB |
TotalSeq™-B1180 anti-Bcl-2 | 100 | ICPG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Bcl-2
Western blot analysis of extracts from THP-1(lane 1), PBMC(l... IHC staining using purified anti-Bcl-2 antibody (clone 100) ... -
Alexa Fluor® 647 anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 488 anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
PE anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
Brilliant Violet 421™ anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
TotalSeq™-Bn1180 anti-Bcl-2
-
TotalSeq™-B1180 anti-Bcl-2
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