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Brilliant Violet 421™ anti-human B7-H4 Antibody

Pricing & Availability
Clone
MIH43 (See other available formats)
Regulatory Status
RUO
Other Names
VTCN1, B7h.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH43_BV421_B7-H4_Antibody_1_110524
Human B7-H4 transfected P815 cells were stained with anti-human B7-H4 (clone MIH43) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • MIH43_BV421_B7-H4_Antibody_1_110524
    Human B7-H4 transfected P815 cells were stained with anti-human B7-H4 (clone MIH43) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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358121 25 tests 155€
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358122 100 tests 326€
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Description

B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) includes immunofluorescence2.

Application References
  1. Quandt D, et al. 2011. Clin. Cancer Res. 17:3100.
  2. Smith JB, et al. 2014. Gynecol. Oncol. 134:181. (FC, IF)

Antigen Details

Structure
Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain
Distribution

Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian

Function
Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Antigen References
  1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
  2. Chen C, et al. 2012. J. Immunother. 35:354.
  3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
  4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748.
Gene ID
79679 View all products for this Gene ID
UniProt
View information about B7-H4 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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