Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1) Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length human PD-L1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region⁵. Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections¹. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). 

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications⁷.

Application References

1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
4. Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
5. Haile, S et al. 2013. J. Immunol. 191:2829.
6. RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
7. Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.

Product Citations

1. Saitoski K, et al. 2022. J Biol Chem. 298:102096. PubMed
2. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
3. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
4. Shenoy GN, et al. 2021. Clin Transl Immunology. 10:e1246. PubMed
5. Nduom E, et al. 2016. Neuro Oncology. 18: 195 - 205. PubMed
6. Mohan N, et al. 2022. Front Immunol. 13:918528. PubMed
7. Ferrando–Martinez S, et al. 2019. JHEP Rep. 0.159722222. PubMed
8. Perdigoto AL, et al. 2022. JCI Insight. 7:. PubMed
9. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
10. Singh S, et al. 2022. Int J Mol Sci. 23:. PubMed
11. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
12. Chakravorty S, et al. 2019. Cancer Res. 79:6010. PubMed
13. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
14. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
15. AlHossiny M, et al. 2016. Cancer Res . 76: 3376 - 3386. PubMed
16. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
17. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
18. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
19. Maurer DM, et al. 2020. Cancer Immunol Res. 8:1554. PubMed

RRID

AB_2563852 (BioLegend Cat. No. 329713)
AB_2563852 (BioLegend Cat. No. 329714)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells

Function

CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.

Ligand/Receptor

PD-1 (PDCD1)

Cell Type

B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells

Biology Area

Cancer Biomarkers, Costimulatory Molecules, Immunology

Molecular Family

Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Gene ID

29126 View all products for this Gene ID

UniProt

View information about CD274 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis