- Clone
- E11 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 204
- Other Names
- C3b/C4b receptor, complement receptor type 1 (CR1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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333415 | 25 tests | 155€ | ||||
333416 | 100 tests | 326€ |
CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
- RRID
-
AB_3068242 (BioLegend Cat. No. 333415)
AB_3068242 (BioLegend Cat. No. 333416)
Antigen Details
- Structure
- Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
- Distribution
-
Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells
- Function
- Adhesion, mediate phagocytosis, inhibit T-cell proliferation
- Ligand/Receptor
- C3b, iC3, C4b, iC4
- Cell Type
- B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699 - Gene ID
- 1378 View all products for this Gene ID
- UniProt
- View information about CD35 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD35 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD35 | E11 | FC,IP,IHC |
FITC anti-human CD35 | E11 | FC |
PE anti-human CD35 | E11 | FC,SB |
TotalSeq™-A0167 anti-human CD35 | E11 | PG |
TotalSeq™-C0167 anti-human CD35 | E11 | PG |
TotalSeq™-B0167 anti-human CD35 | E11 | PG |
Biotin anti-human CD35 | E11 | FC |
Brilliant Violet 421™ anti-human CD35 | E11 | FC |
TotalSeq™-D0167 anti-human CD35 | E11 | PG |
APC anti-human CD35 | E11 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD35
Human peripheral blood lymphocytes stained purified E11, fol... -
FITC anti-human CD35
Human peripheral blood lymphocytes stained with E11 FITC -
PE anti-human CD35
Human peripheral blood lymphocytes stained with E11 PE Confocal image of human lymph node sample acquired using the... Confocal image of human lymph node sample acquired using the... -
TotalSeq™-A0167 anti-human CD35
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TotalSeq™-C0167 anti-human CD35
-
TotalSeq™-B0167 anti-human CD35
-
Biotin anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0167 anti-human CD35
-
APC anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu...
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