- Clone
- ZET (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![ZET_BV510_XCR1_Antibody_FC_1_122214 ZET_BV510_XCR1_Antibody_FC_1_122214](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/ZET_BV510_XCR1_Antibody_FC_1_122214.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c PE, CD8α APC, and XCR1 (clone ZET) Brilliant Violet 510™ (top) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (bottom). Data shown was gated on the CD11c+ population. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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148218 | 50 µg | 234€ |
XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
- Application References
-
- Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
- Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
- Product Citations
-
- RRID
-
AB_2565231 (BioLegend Cat. No. 148218)
Antigen Details
- Structure
- Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
- Distribution
-
Subset of CD8α+ conventional dendritic cells.
- Function
- Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
- Ligand/Receptor
- XCL1 and XCL2.
- Cell Type
- Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411. - Gene ID
- 23832 View all products for this Gene ID 301086 View all products for this Gene ID
- UniProt
- View information about XCR1 on UniProt.org
Related FAQs
Other Formats
View All XCR1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse/rat XCR1 | ZET | FC |
Purified anti-mouse/rat XCR1 | ZET | FC,IHC-F |
APC anti-mouse/rat XCR1 | ZET | FC |
PerCP/Cyanine5.5 anti-mouse/rat XCR1 | ZET | FC |
FITC anti-mouse/rat XCR1 | ZET | FC |
Biotin anti-mouse/rat XCR1 | ZET | FC |
Alexa Fluor® 647 anti-mouse/rat XCR1 | ZET | FC |
Brilliant Violet 421™ anti-mouse/rat XCR1 | ZET | FC |
Brilliant Violet 510™ anti-mouse/rat XCR1 | ZET | FC |
Brilliant Violet 650™ anti-mouse/rat XCR1 | ZET | FC |
APC/Cyanine7 anti-mouse/rat XCR1 | ZET | FC |
Brilliant Violet 785™ anti-mouse/rat XCR1 | ZET | FC |
TotalSeq™-A0568 anti-mouse/rat XCR1 | ZET | PG |
TotalSeq™-C0568 anti-mouse/rat XCR1 | ZET | PG |
TotalSeq™-B0568 anti-mouse/rat XCR1 Antibody | ZET | PG |
PE/Dazzle™ 594 anti-mouse/rat XCR1 | ZET | FC |
Brilliant Violet 605™ anti-mouse/rat XCR1 | ZET | FC |
Spark UV™ 387 anti-mouse/rat XCR1 | ZET | FC |
PE/Cyanine7 anti-mouse/rat XCR1 | ZET | FC |
Spark PLUS UV™ 395 anti-mouse/rat XCR1 | ZET | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Purified anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
APC anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
PerCP/Cyanine5.5 anti-mouse/rat XCR1
Mouse spleen cells, from collagenase-digested C57BL/6, were ... -
FITC anti-mouse/rat XCR1
Mouse spleen cells, from collagenase-digested C57BL/6, were ... -
Biotin anti-mouse/rat XCR1
Mouse spleen cells, from collagenase-digested C57BL/6, were ... -
Alexa Fluor® 647 anti-mouse/rat XCR1
Mouse spleen cells, from collagenase-digested C57BL/6, were ... -
Brilliant Violet 421™ anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Brilliant Violet 510™ anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Brilliant Violet 650™ anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
APC/Cyanine7 anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Brilliant Violet 785™ anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
TotalSeq™-A0568 anti-mouse/rat XCR1
-
TotalSeq™-C0568 anti-mouse/rat XCR1
-
TotalSeq™-B0568 anti-mouse/rat XCR1 Antibody
-
PE/Dazzle™ 594 anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Brilliant Violet 605™ anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Spark UV™ 387 anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
PE/Cyanine7 anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st... -
Spark PLUS UV™ 395 anti-mouse/rat XCR1
Cells from collagenase-digested C57BL/6 mouse spleen were st...
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