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Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads

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CV_GMP_CD3_CD28_Human_T_Cell_Activation_Beads_image1_070224
PBMCs were stimulated with Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads (cat#: 422605, bar with vertical stripes) at a 1:1 bead-to-cell ratio. This condition was compared to BioLegend Human CD3/CD28 T Cell Activation beads (cat#: 422603, bar with checkered pattern) and a similar product from a Competitor (bar with diagonal stripes). PBMCs were activated by plating 1x106 cells in a 12 well plate and cultured for a total of 10 days. Fold expansion over D0 was calculated at D10 and plotted for each activation condition.
  • CV_GMP_CD3_CD28_Human_T_Cell_Activation_Beads_image1_070224
    PBMCs were stimulated with Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads (cat#: 422605, bar with vertical stripes) at a 1:1 bead-to-cell ratio. This condition was compared to BioLegend Human CD3/CD28 T Cell Activation beads (cat#: 422603, bar with checkered pattern) and a similar product from a Competitor (bar with diagonal stripes). PBMCs were activated by plating 1x106 cells in a 12 well plate and cultured for a total of 10 days. Fold expansion over D0 was calculated at D10 and plotted for each activation condition.
  • CV_GMP_CD3_CD28_Human_T_Cell_Activation_Beads_image2_070224
    (A) PBMCs were stimulated with Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads (cat#: 422605, bar with vertical stripes) at a 1:1 bead-to-cell ratio. This condition was compared to BioLegend Human CD3/CD28 T Cell Activation beads (cat#: 422603, bar with checkered pattern) and a similar product from a Competitor (bar with diagonal stripes). PBMCs were activated by plating 1x106 cells in a 12 well plate and cultured for a total of 10 days. Cell phenotype was analyzed by flow cytometry using the following definitions: T cell is 7-AAD-/CD3+/CD56-/CD19-; NK cell is 7-AAD-/CD56+/CD3-/CD19-; B cell is 7-AAD-/CD19+/CD3-/CD56-. (B) CD3+ T cells were further analyzed for phenotypic and activation markers. All T cells were defined by 7-AAD-/CD3+ gating.
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422605 0.5 mL 351€
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422606 2.5 mL 1231€
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Description

Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads allow for convenient in vitro or ex vivo activation of human T cells without the need for antigen-presenting cells. Human T cells are stimulated with anti-CD3 and anti-CD28 antibodies coated on superparamagnetic microbeads. Simply add the beads directly to isolated T cells or to PBMCs at a 1:1 bead-to-cell ratio for robust T cell activation. Excess antibody-coated beads can be removed from the cell suspension by the use of a magnetic separator (some residual beads may be retained in the cell suspension). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Activation and Expansion Tools are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Low endotoxin level (< 1 EU/mL)
  • Manufactured under serum-free conditions
  • Bioburden/sterility and mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Formulation
Beads are resuspended in phosphate buffer solution containing 0.1% recombinant HSA.
Endotoxin Level
< 1 EU/mL
Preparation
The antibodies were purified by affinity chromatography and conjugated with magnetic beads under optimized conditions. The final product is a GMP, ready to use solution.
Concentration
4.0 x 107 ± 10% beads/mL
Storage & Handling
4°C (2-8 °C)
Application

Cell Culture - Quality tested

Activ - Verified in house
Recommended Usage

GMP CD3/CD28 Human T Cell activation beads should be plated with cells at a 1:1 bead-to-cell ratio.

Application Notes

This product is designed for the in vitro or ex vivo expansion of human T cells.

 

Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads are a ready to use solution. There is no need to dilute the product prior to use in cell culture.

 

Suggested culture protocol:

  1. Prepare T Cell Complete Media (TCCM) using sterile, filtered IMDM with 5% final concentration of serum and 200 IU/mL of human IL-2.
  2. Prepare cells at a concentration of 1 x 106 per mL of TCCM.
  3. Wash 25 µL of Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads to a 5 mL polypropylene tube with 1 mL TCCM.
  4. Apply tube containing beads to a magnet (MojoSort magnet, cat#: 480019) for 2 minutes. Decant supernatant and remove tube from magnet.
  5. Resuspend beads in 1 mL of cell suspension and plate in a 12 well cell culture plate.
  6. Allow cells to expand at 37°C and 5% CO2. At D4 and D7, count cells and resuspend at 0.5 x 106 cells per mL.
  7. Harvest cells at D10 or D11 for further analysis.

This product was optimized on isolated CD3+ T cells and PBMCs. Alternate sources of T cells may require protocol optimization.

Disclaimer

BioLegend Cell-Vive™ GMP Activation and Expansion tools are for research use only. Suitable for ex vivo cell processing. Not for use in diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02/20/2025

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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