- Regulatory Status
- RUO
- Other Names
- ELISA assay diluent, ELISA diluent
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- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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421203 | 100 mL | 57€ |
ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.
Product DetailsProduct Details
- Storage & Handling
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This solution can be stored unopened between 2°C and 8°C. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage.
Warm ELISA reagents to room temperature (18-25°C) before use. - Application
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ELISA
- Recommended Usage
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Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4.
PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 µm filter, pH to 7.4 - Application References
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- Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed
- Product Citations
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Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- I ran out of assay diluent provided with ELISA MAX™ Deluxe set.
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You can order more assay diluent using Cat. No. 421203.
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