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GMP Recombinant Human FN-1 (carrier-free)

Pricing & Availability
Other Names
Fibronectin, FN, FN1, Cold-insoluble globulin, CIG, Large external transformation-sensitive protein, LETS
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GMP_RECOM_Human_FN-1_CF_062923
Immobilized GMP recombinant human FN-1 supports the adhesion of mouse L929 cells in a dose dependent manner. The ED50 for this effect is 15 – 60 ng/mL.
  • GMP_RECOM_Human_FN-1_CF_062923
    Immobilized GMP recombinant human FN-1 supports the adhesion of mouse L929 cells in a dose dependent manner. The ED50 for this effect is 15 – 60 ng/mL.
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775314 25 µg 196€
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775316 100 µg 636€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Fibronectin (FN) is an adhesive glycoprotein found as insoluble components of the extracellular matrix (ECM) or as soluble dimeric forms in plasma. Fibronectin binds chondroitin sulfate, collagen, fibrin, heparin, and many integrin receptors through which it mediates multiple cellular signaling pathways. Conversion of soluble fibronectin to fibronectin fibrils is initiated by binding to cell surface integrins. Fibronectin is involved in cell adhesion, cell motility, wound healing and maintenance of cell morphology. It also participates in osteoblast compaction and is essential for osteoblast mineralization. Fibronectin is thought to play a role in platelet thrombus formation and other diseases associated with thrombosis including atherosclerosis and cardiac repair following a myocardial infarct. Fibronectin plays an important role in coordinating interaction between the ECM and cancer cells in tumors. Its upregulation enhances cancer cell survival, proliferation, and invasion. Fibronectin monomers consist of three types of homologous repeats, type I, II and III domains. A fragment from the first type III repeat, known as Anastellin, binds to fibronectin and induces fibril formation named superfibronectin. Superfibronectin exhibits enhanced adhesive properties and inhibits tumor growth, angiogenesis, and metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human NF-1, amino acid (Gln32-Pro1991) (Accession: # BC117176) with a C-terminal 8His-GGQ tag was expressed in 293E cells.
Molecular Mass
The 1973 amino acid recombinant protein has a predicted molecular mass of approximately 217 kD. The DTT-reduced and non-reduced protein migrates at approximately 180 – 220 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.1 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
0.5 mg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized recombinant human FN-1 supports the adhesion of mouse L929 cells in a dose dependent manner. The ED50 for this effect is 15 – 60 ng/mL.
Application

Bioassay
Cell Culture
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Monomer
Distribution

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial, and other cell types, is deposited as fibrils in the extracellular matrix.

Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin
Interaction
Cell surface, collagen, fibrin, heparin, DNA, and actin
Ligand/Receptor
Integrins α5β1, α4β1, α4β7, α9β1, αvβ3; non-integrin receptors TLR-4, CD44, Syndecan-4, collagen, fibrin, heparin, DNA, and actin
Bioactivity
FN-1 supports the adhesion of mouse L929 cells.
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience Cell Markers
Antigen References
  1. Schwarz-Linek U, et al. 2004. Mol Microbiol. 52:631.
  2. Nagai Y, et al. 2006. Immunity. 24:801.
  3. White ES, et al. 2008. J. Pathol. 216:1.
  4. Adams JC, et al. 2015. Cell Adh Migr. 9:22.
  5. Kraft S, et al. 2016. J. Mol. Med. 94:567.
  6. Speziale P, et al. 2019. Cells. 8:1516.
  7. Wirth F, et al. 2020. Cells. 9:2717.
Gene ID
2335 View all products for this Gene ID
UniProt
View information about FN-1 on UniProt.org
Go To Top Version: 1    Revision Date: 07/19/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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