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Biotinylated Recombinant Human VEGFR2-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Kinase insert domain receptor (KDR), Tyrosin kinase growth factor receptor, homolog of FLK1 (FLK1), VEGFR
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Product Citations
publications
a. Biotin-Recom_Human-KDR-Fc-Chimera_1_102424
When recombinant human VEGF-165 (Cat. No. 583706) is immobilized at 1 µg/mL, recombinant biotinylated human VEGFR2 (KDR) binds in a dose-dependent manner with ED50 of 3 – 18 ng/mL.
  • a. Biotin-Recom_Human-KDR-Fc-Chimera_1_102424
    When recombinant human VEGF-165 (Cat. No. 583706) is immobilized at 1 µg/mL, recombinant biotinylated human VEGFR2 (KDR) binds in a dose-dependent manner with ED50 of 3 – 18 ng/mL.
  • b. Biotin-Recom_Human-KDR-Fc-Chimera_2_102424
    Stability Testing for Biotinylated Recombinant Human VEGFR2 (KDR). Biotinylated recombinant human VEGFR2 (KDR) was aliquoted in PBS, pH 7.2. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human VEGF-165. The ED50 for this effect is 3 – 18 ng/mL.
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570754 25 µg 244€
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570756 100 µg 568€
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Description

The VEGF family includes five members, VEGFA, VEGFB, VEGFC, VEGFD, and placenta growth factor (PlGF). Structurally, these growth factors are homodimers, although heterodimers have been described that are composed of VEGFA and PIGS. There are three VEGF receptor tyrosine kinases: VEGFR1 (Flt1), VEGFR2 (KDR/Flk1), and VEGFR3 (Flt4). VEGFR1 is expressed on monocytes and macrophages, VEGFR2 on vascular endothelial cells, and VEGFR3 on lymphatic endothelial cells. VEGFR2 binds to proteolytically processed VEGFC and VEGFD. VEGFR2 is the major mediator of the signaling cascades that regulate endothelial cell functions including proliferation, migration and differentiation. Soluble VEGFR2 was initially identified in mouse and human plasma resulting from an endothelial cell surface proteolytic cleavage. VEGFA stimulates ADAM17- dependent shedding of VEGFR2. In addition, its shedding is stimulated by phorbol ester (PMA) and the calcium ionophore, ionomycin. Soluble VEGFR1 and VEGFR2 act as decoy receptors for VEGFA regulating the availability of VEGFA. Upon ligand binding, VEGFR1 and VEGFR2 can dimerize as homodimers or heterodimers, contributing to various pathways and downstream effects. In addition, alternative splicing of VEGFR2 produces soluble VEGFR2 that binds VEGFC and subsequently prevents lymphangiogenesis. It has been reported that decreased levels of VEGFR2 in the plasma of preeclamptic women may serve as a marker of endothelial cell dysfunction.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Biotinylated Recombinant Human KDR (VEGFR2), amino acid (Ala20-Glu764) (Accession # P35968.2), with a linker, a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag was site-specifically biotinylated by enzyme BirA, was expressed in 293E cells.
Molecular Mass
The 997 amino acid recombinant protein has a predicted molecular mass of approximately 111.73 kD. The DTT-reduced and non-reduced protein migrates at approximately 130 – 240 kD and 240 – 400 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ala.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. Stock solutions should be prepared at no less than 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human VEGF-165 (Cat. No. 583706) is immobilized at 1 µg/mL, biotinylated recombinant human VEGFR2 binds in a dose-dependent manner with ED50 of 3 – 18 ng/mL.
Application

Biossay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Soluble VEGFR2 is derived from endothelial cells.

Function
The main function of VEGFR2 is angiogenesis in physiological and pathological conditions. The transcription of VEGFR2 is regulated by endogenous VEGFA. Soluble VEGFR2 is a decoy receptor for VEGFA and VEGFC.
Interaction
Endothelial cells
Ligand/Receptor
VEGFA, VEGFC
Bioactivity
The bioactivity was measured by immobilized recombinant human VEGF-165 binding biotinylated recombinant human VEGFR2 in a dose-dependent manner using the functional ELISA.
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokine/Chemokine Receptors, Soluble Receptors
Antigen References
  1. DiSalvo J, et al. 1995. J Biol Chem. 270:7717-23.
  2. McColl BK, et al. 2003. J Exp Med. 198:863-8.
  3. Albuquerque RJ, et al. 2009. Nat Med. 15:1023-30.
  4. Guangqi E, et al. 2012. J Biol Chem. 287:3029-41.
  5. Koch S and Claesson-Welsh 2012. Cold Spring Harb Perspect Med. 2:a006502.
  6. Munaut C, et al. 2012. Plos One. 7:e33475.
  7. Wehland M, et al. 2013. Int J Mol. Sci. 14:9338.
Gene ID
3791 View all products for this Gene ID
UniProt
View information about VEGFR2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/14/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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