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LEGEND MAX™ Human IL-6 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-6 Pre-coated ELISA Kit
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Product Citations
publications
human il-6_010510
  • human il-6_010510
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430507 1 Pre-coated Plate 328€
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Description

Human IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hybrid­oma growth factor, and CTL differentiation factor, etc.) plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, and in hepatocytes it induces acute phase reactants. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis.

BioLegend’s LEGEND MAX™ Human IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-6 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-6 ELISA Kit is specifically designed for the accurate quantitation of Human IL-6 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IL-6 Pre-coated 96-well Strip Microplate
  • Human IL-6 Dectection Antibody
  • Human IL-6 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Product Citations
  1. Liu HW, et al. 2020. Gerontology. 66:562. PubMed
  2. Hasebe R, et al. 2022. J Exp Med. 219: . PubMed
  3. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2200263. PubMed
  4. Chen J, et al. 2020. Mol Med Rep. 1.144444444. PubMed
  5. Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed
  6. Liang Y, et al. 2018. Oncotarget. 9:14862. PubMed
  7. Kusmiwiyati A, et al. 2018. J Taibah Univ Med Sci. 13:129. PubMed
  8. Spittaels KJ, et al. 2021. iScience. 24:102575. PubMed
  9. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  10. Sreenivasan L, et al. 2022. Cells. 11:. PubMed
  11. Neidlinger-Wilke C, et al. 2021. Eur Cell Mater. 42:1. PubMed
  12. Devadoss D, et al. 2022. iScience. 25:104685. PubMed
  13. Gholamnezhadjafari R, et al. 2017. Innate Immun. 23:440. PubMed
  14. Hubbard T, et al. 2015. Sci Rep. 5: 12689. PubMed
  15. Dudau M, et al. 2021. Front Pharmacol. 12:737571. PubMed
  16. Rana T, et al. 2021. PLoS One. 16:e0246393. PubMed
  17. Sreenivasan L, et al. 2020. Cell Death Dis. 1.177083333. PubMed
  18. Holtkamp C, et al. 2019. PLoS One. 14:e0217269. PubMed
Sensitivity
1.6 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 12/13/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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