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The LEGENDplex™ Mouse B cell Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel focuses on 13 key biomarkers involved in B cell function, activation, and survival. It allows for simultaneous quantification of 13 mouse targets such as IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, and IL-10. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
The LEGENDplex™ Mouse B cell Panel is configured as shown below depending on sample types and required dilutions:
|
Catalog No. |
Plex Size |
Targets |
Recommended Sample Type |
Recommended Dilution Factor |
|
740818 740819 |
13-plex |
IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, IL-10 |
Tissue Culture* |
Varies |
|
740826 740827 |
12-plex |
IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BCMA, sCD40L, IL-10 |
Serum, Plasma |
2 |
|
Tissue Culture |
Varies |
|||
|
740982 740983 |
1-plex |
BAFF |
Serum, Plasma |
25 |
|
Tissue Culture |
Varies |
|||
|
740824 740825 |
3-plex |
IL-12p70, TNF-α, IFN-γ |
Serum, Plasma |
2 |
|
Tissue Culture |
Varies |
|||
|
740822 740823 |
5-plex |
IL-4, IL-6, IL-2, TNF-α, IL-13 |
Serum, Plasma |
2 |
|
Tissue Culture |
Varies |
|||
|
740820 740821 |
8-plex |
IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, IL-13, IFN-γ |
Serum, Plasma |
2 |
|
Tissue Culture |
Varies |
|||
|
740828 740829 |
2-plex |
Free Active TGF-β1, IL-10 |
Serum, Plasma |
2 |
|
Tissue Culture |
Varies |
*Note: Serum and plasma are not the recommended sample type. Refer to Sample Dilution section in the manual for details.
The LEGENDplex™ Mouse B cell Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel. This assay is for research use only.
Product DetailsKit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- Capture Beads
- Detection Antibodies
- Standard Cocktail, Lyophilized
- Matrix C1, Lyophilized
- SA-PE
- Assay Buffer
- Wash Buffer
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Mouse
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Aluminum foil
- Absorbent pads or paper towels
- Sonicator
- Plate Shaker
- Tabletop centrifuge
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Manual
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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