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Pacific Blue™ anti-mouse Ly108 Antibody

Pricing & Availability
Clone
330-AJ (See other available formats)
Regulatory Status
RUO
Other Names
SLAM family receptor, SLAMF6, TCOM, SF2000, KALI, SF-3, NTBA, NTB-A, NK-T-B antigen
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_330-AJ_PB_092109
C57BL/6 splenocytes stained with 330-AJ Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
  • A_330-AJ_PB_092109
    C57BL/6 splenocytes stained with 330-AJ Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
  • B_330-AJ_PB_092109
    C57BL/6 splenocytes double stained with mouse IgG2a isotype control Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
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134608 100 µg 259€
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Description

Mouse Ly108, also known as SLAMF6 and NTB-A (NK cell, T cell, B cell antigen), is one of the members in The Signaling Lymphocytic Activation Molecule (SLAM) family of immune receptors. It is expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes. Homophilic interaction of Ly108 is involved in augmenting cytotoxicity of NK cells. Ly108 has been shown to function on NK cells by augmenting cytotoxicity. It was reported that Ly108 plays an important role in CD4+ T cell responses and innate immunity to bacteria and parasites. In a mouse with a targeted disruption of the Ly108 gene, CD4+ T cells and innate responses are defective. SLAM family of receptors has been implicated in the pathophysiology of autoimmunity. For instance, Ly108 is strongly linked to lupus susceptibility in mice. Ly108 may censor self-reactive B cells as a potential regulator of tolerance checkpoints, safeguarding against autoimmunity. Therefore, Ly108 serves as a regulator of both innate and adaptive immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application References
  1. Fu GF, et al. 2012. Int Immunol. 24:197. PubMed.
Product Citations
  1. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  2. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  3. Zander R, et al. 2022. Immunity. 55:475. PubMed
  4. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  5. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  6. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  7. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  8. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
RRID
AB_2188093 (BioLegend Cat. No. 134608)

Antigen Details

Structure
A glycoprotein of the signaling lymphocytic activation molecule family of cell surface receptor. It is a novel member of the CD2 subfamily of the Ig superfamily.
Distribution

Expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes.

Function
Play an important role in CD4+ T cell responses, innate immunity to bacteria and parasites, and NK cell function.
Ligand/Receptor
Ly108
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Howie D, et al. 2005. J. Immunology. 174 (10):5931
2 Kumar KR, et al. 2006. Science. 312(5780):1665
3. Zhong MC, et al. 2008. J. Biol. Chem. 283 (28):19255
4. Peck SR et al. 2000. Immunogenetics. 52:63

Gene ID
30925 View all products for this Gene ID
UniProt
View information about Ly108 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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