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PE anti-mouse CD62L Antibody

Pricing & Availability
Clone
W18021D (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18021D_PE_CD62L_Antibody_080520
C57BL/6 splenocytes were stained with anti-mouse CD44 PE/Cyanine7 and anti-mouse CD62L (clone W18021D) PE (left) or Rat IgG2a, κ isotype control PE (right). Dot plots are gated on CD4 positive cells.
  • W18021D_PE_CD62L_Antibody_080520
    C57BL/6 splenocytes were stained with anti-mouse CD44 PE/Cyanine7 and anti-mouse CD62L (clone W18021D) PE (left) or Rat IgG2a, κ isotype control PE (right). Dot plots are gated on CD4 positive cells.
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161203 25 µg 55€
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161204 100 µg 137€
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD62L recombinant protein (39-332)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  2. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  3. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
RRID
AB_2876576 (BioLegend Cat. No. 161203)
AB_2876576 (BioLegend Cat. No. 161204)

Antigen Details

Structure
CD62L is a type I transmembrane glycoprotein with 74-95 kD.
Distribution

CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.

Function
CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Rosen S. 2004. Annu. Rev. Immunol. 22:129
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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