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PE/Cyanine7 anti-human IL-31 Antibody

Pricing & Availability
Clone
1D10B31 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-31
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1D10B31_PE-Cyanine7_IL-31_Antibody_120622
Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303). Cells were stained with anti-human CD3 APC and then fixed and permeabilized. Cells were then intracellularly stained with anti-human IL-31 (clone 1D10B31) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 isotype control (right).
  • 1D10B31_PE-Cyanine7_IL-31_Antibody_120622
    Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303). Cells were stained with anti-human CD3 APC and then fixed and permeabilized. Cells were then intracellularly stained with anti-human IL-31 (clone 1D10B31) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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659609 25 tests 141€
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659610 100 tests 329€
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Description

IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune response. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human IL-31 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2928022 (BioLegend Cat. No. 659609)
AB_2928022 (BioLegend Cat. No. 659610)

Antigen Details

Structure
161 aa with with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution
Secreted by activated CD4 T cells.
Function
IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand/Receptor
Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Cell Type
T cells, Eosinophils
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.

Gene ID
386653 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org
Go To Top Version: 1    Revision Date: 12/06/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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