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PE/Cyanine7 anti-mouse CD357 (GITR) Antibody

Pricing & Availability
Clone
YGITR 765 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF18, AITR
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YGITR765_PECy7_091207
C57BL/6 Mouse splenocytes stained with YGITR765 PE/Cyanine7
  • YGITR765_PECy7_091207
    C57BL/6 Mouse splenocytes stained with YGITR765 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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120222 100 µg 278€
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Description

GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse GITR protein and Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 120216).

Application References
  1. Cobbold SP, et al. 2004. J. Immunol. 172:6003.
  2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
Product Citations
  1. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  2. An J, et al. 2022. iScience. 25:103570. PubMed
  3. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  4. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  5. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
RRID
AB_528907 (BioLegend Cat. No. 120222)

Antigen Details

Structure
TNF receptor superfamily member
Distribution

Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.

Function
Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand/Receptor
GITR ligand (TNFSF18)
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613
3. Kanamaru F, et al. 2004. J. Immunol. 172:613
4. Shimizu J, et al. 2002. Nat. Immunol. 3:135
5. Stephens GL, et al. 2004. J. Immunol. 173:5008
6. McHugh RS, et al. 2002. Immunity 16:311

Gene ID
21936 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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