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PE/Cyanine7 anti-mouse Ly-49C/F/I/H Antibody

Pricing & Availability
Clone
14B11 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-49 inhibitory receptors C, F and I and the activating receptor H
Isotype
Syrian Hamster IgG
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Product Citations
publications
14B11_PECyanine7_Ly-49CFIH_Antibody_1_101723
C57/BL6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse CD49b (clone DX5) APC, and anti-mouse Ly-49C/F/I/H (clone 14B11) PE/Cyanine7 (left) or Syrian Hamster IgG Isotype Ctrl PE/Cyanine7 (right).
  • 14B11_PECyanine7_Ly-49CFIH_Antibody_1_101723
    C57/BL6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse CD49b (clone DX5) APC, and anti-mouse Ly-49C/F/I/H (clone 14B11) PE/Cyanine7 (left) or Syrian Hamster IgG Isotype Ctrl PE/Cyanine7 (right).
Compare all formats See PE/Cyanine7 spectral data
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108209 25 µg 128€
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108210 100 µg 296€
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Description

The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8+ cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
IL-2-activated killer cells (LAK) from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: induction of redirected lysis1, and blocking2 of the binding of H-2d lymphoblasts to transfectants expressing Ly-49C, Ly-49F, and Ly-49I.

Application References
  1. Corral L, et al. 1999. Hybridoma 18:359.
  2. Hanke T, et al. 1999. Immunity 11:67.
Product Citations
  1. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
RRID
AB_2728130 (BioLegend Cat. No. 108209)
AB_2728130 (BioLegend Cat. No. 108210)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested for the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ly-49 family, 110 kD
Distribution

NK and NK-T cells, some CD8+ T cells

Function
NK cell activation or inhibition
Ligand/Receptor
MHC Class I Molecules
Cell Type
NK cells, NKT cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Ryan JC, et al. 1997. Immunol. Rev. 155:79.
2. Mason LH, et al. 1997. J. Immunol. 159:4187.
3. Lanier LL. 1998. Ann. Rev. Immunol. 16:359.
4. Yokoyama W, et al. 1993. Ann. Rev. Immunol. 11:613.

Gene ID
16634 View all products for this Gene ID
UniProt
View information about Ly-49C/F/I/H on UniProt.org
Go To Top Version: 1    Revision Date: 03/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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