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- Clone
- KJ1-26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DO11.10 Clonotypic T Cell Receptor
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
DO11.10 T-hybridoma cells were stained with TCR DO11.10 (clone KJ1-26) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 118515 | 25 µg | 100€ | ||||
| 118516 | 100 µg | 268€ | ||||
DO11.10 transgenic TCR is specific for the chicken ovalbumin peptide (a.a. 323-339) presented on the I-Ad MHC class II complex. KJ1-26 antibody clone recognizes the DO11.10 TCR and enables identification of the DO11.10 TCR positive T-cells in the DO11.10 TCR transgenic mouse model. KJ1-26 antibody clone was reported to block the antigen response of DO11.10 T cell hybridomas in vitro.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- DO11.10 T hybridoma cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application References
-
- Haskins K, et al. 1983. J. Exp. Med. 157:1149.
- Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
- Product Citations
-
- RRID
-
AB_2562392 (BioLegend Cat. No. 118515)
AB_2562392 (BioLegend Cat. No. 118516)
Antigen Details
- Structure
- 80-90 kD TCR expressed on DO11.10 T hybridomas
- Distribution
-
Clonotypic TCR
- Ligand/Receptor
- Ovalbumin peptide (aa 323-339) in the context of mouse MHC class II I-Ad
- Biology Area
- Immunology, Adaptive Immunity
- Molecular Family
- TCRs
- Antigen References
-
1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Marrack P, et al. 1983. J. Exp. Med. 158:1635. - Gene ID
- 21473 View all products for this Gene ID
- UniProt
- View information about TCR DO11.10 on UniProt.org
Other Formats
View All TCR DO11.10 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse TCR DO11.10 | KJ1-26 | FC |
| PE anti-mouse TCR DO11.10 | KJ1-26 | FC |
| PerCP/Cyanine5.5 anti-mouse TCR DO11.10 | KJ1-26 | FC |
| PE/Cyanine7 anti-mouse TCR DO11.10 | KJ1-26 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse TCR DO11.10
DO11.10 T-hybridoma cells stained with KJ1-26 FITC -
PE anti-mouse TCR DO11.10
DO.11.10 cell line stained with KJ1-26 PE -
PerCP/Cyanine5.5 anti-mouse TCR DO11.10
DO11.10 cells stained with KJ1-26 PerCP/Cyanine5.5 -
PE/Cyanine7 anti-mouse TCR DO11.10
DO11.10 T-hybridoma cells were stained with TCR DO11.10 (clo...
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