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PerCP/Cyanine5.5 anti-human CD4 Antibody

Pricing & Availability
Clone
A161A1 (See other available formats)
Regulatory Status
RUO
Other Names
T4/Leu-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A161A1_PerCPCy5.5_CD4_Antibody_FC_050515
Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • A161A1_PerCPCy5.5_CD4_Antibody_FC_050515
    Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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357413 25 tests 128€
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357414 100 tests 287€
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Description

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD4 T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Product Citations
  1. Kim S, et al. 2023. Front Immunol. 14:1062365. PubMed
  2. Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed
  3. Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed
  4. Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed
  5. Idiz UO, et al. 2022. J Med Virol. 94:4744. PubMed
  6. Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed
  7. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  8. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  9. Yang W, et al. 2019. Nat Med. 25:767. PubMed
  10. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  11. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  12. Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
RRID
AB_2565666 (BioLegend Cat. No. 357413)
AB_2565666 (BioLegend Cat. No. 357414)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Cell Type
T cells, Monocytes, Macrophages, NKT cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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