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PerCP/Cyanine5.5 anti-human IL-2 Antibody

Pricing & Availability
Clone
MQ1-17H12 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
MQ1-17H12_PerCPCyanine55_IL-2_Antibody_1_112024
PMA + ionomycin-stimulated (6 hours, in the presence of monesin) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-2 (clone MQ1-17H12) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
  • MQ1-17H12_PerCPCyanine55_IL-2_Antibody_1_112024
    PMA + ionomycin-stimulated (6 hours, in the presence of monesin) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-2 (clone MQ1-17H12) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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500321 25 tests 141€
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500322 100 tests 306€
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The IL-2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.

Clone MQ1-17H12 cross-reacts to Cat15

Application References
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed
  2. Taus E, et al. 2023. Front Immunol. 14:1100594. PubMed
  3. Taus E, et al. 2023. J Transl Med. 21:374. PubMed
  4. Liu F, et al. 2016. PLoS Pathog. 12: 1005663. PubMed
  5. Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed
  6. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  7. Awasthi S, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006141. PubMed
  8. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
  9. Fourcade J, et al. 2010. J Exp Med. 207:2175. PubMed
  10. Fang F, et al. 2022. JCI Insight. 7:. PubMed
  11. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Souza M, et al. 2012. J Immunol. 188:5166. PubMed
  13. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  14. Nomura T, et al. 2012. J Virol. 86:6481. PubMed
  15. Chiurchiù V, et al. 2016. Sci Transl Med. 8: 353ra111. PubMed
  16. Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
RRID
AB_2123667 (BioLegend Cat. No. 500321)
AB_2123667 (BioLegend Cat. No. 500322)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 05/23/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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