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PerCP/Cyanine5.5 anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA16A02 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA16A02_PerCP_Cyanine5-5_GranzymeB_Antibody_111723
Human peripheral blood mononuclear cells were stained with CD8 FITC, fixed, permeabilized, and then stained with Granzyme B (clone QA16A02, left) PerCP/Cyanine5.5 or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • QA16A02_PerCP_Cyanine5-5_GranzymeB_Antibody_111723
    Human peripheral blood mononuclear cells were stained with CD8 FITC, fixed, permeabilized, and then stained with Granzyme B (clone QA16A02, left) PerCP/Cyanine5.5 or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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372211 25 tests 141€
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372212 100 tests 306€
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

 

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed
  2. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  3. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  4. Xiong H, et al. 2023. EBioMedicine. 90:104507. PubMed
  5. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  6. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  7. Gao F, et al. 2023. Immunity. 56:864. PubMed
  8. Park SY, et al. 2022. NPJ Vaccines. 7:123. PubMed
  9. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  10. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  11. Valestrand L, et al. 2022. Am J Pathol. . PubMed
  12. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  13. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  14. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  15. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  16. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  17. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2728378 (BioLegend Cat. No. 372211)
AB_2728378 (BioLegend Cat. No. 372212)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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