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PerCP/Cyanine5.5 anti-mouse CD34 Antibody

Pricing & Availability
Clone
HM34 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosialin
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM34_PerCPCyanine55_CD34_Antibody_101818
Mouse fibroblast NIH/3T3 cells were stained with CD34 (clone HM34) PerCP/Cyanine5.5 (filled histogram), or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • HM34_PerCPCyanine55_CD34_Antibody_101818
    Mouse fibroblast NIH/3T3 cells were stained with CD34 (clone HM34) PerCP/Cyanine5.5 (filled histogram), or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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128607 25 µg 141€
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128608 100 µg 306€
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD34 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.

Product Citations
  1. Tao X, et al. 2022. EMBO J. 41:e110439. PubMed
  2. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  3. Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed
  4. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  5. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  6. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  7. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  8. Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
  9. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  10. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  11. Oppezzo A, et al. 2020. J Clin Invest. 130:1377. PubMed
  12. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_1279222 (BioLegend Cat. No. 128607)
AB_1279222 (BioLegend Cat. No. 128608)

Antigen Details

Structure
Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells

Function
Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, and others.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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