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PerCP/Cyanine5.5 anti-mouse FcεRIα Antibody

Pricing & Availability
Clone
MAR-1 (See other available formats)
Regulatory Status
RUO
Other Names
FceRIa, FceRI-α, high affinity IgE receptor
Isotype
Armenian Hamster IgG
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Product Citations
publications
MAR-1_PerCPCyanine55_FceRla_Antibody_022219
Mouse mast cell line MC/9 was stained with FceRIa (clone MAR-1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • MAR-1_PerCPCyanine55_FceRla_Antibody_022219
    Mouse mast cell line MC/9 was stained with FceRIa (clone MAR-1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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134319 25 µg 141€
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134320 100 µg 306€
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Description

FcεRIα is a transmembrane protein belonging to the Ig superfamily. FcεRIα forms a tetrameric complex with one β and two γ-subunits. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. It is abundantly expressed on mast and basophils and up-regulated by the presence of IgE. Following stimulation via FcεRIα, mast cells and basophils release bioactive chemical mediators such as histamine, resulting in the initiation of allergic reactions. Cross linking of the high-affinity receptor for IgE on tissue mast cells triggers immediate hypersensitivity with local symptoms. The MAR-1 monoclonal antibody reacts with the FcεRIα subunit.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: depletion2, immunohistochemistry of frozen sections (OCT embedded2).

Application References
  1. Obata K, et al. 2007. Blood 110:913 (FC)
  2. Sokol CL, et al. 2008. Nat. Immunol. 9:310 (FC, Deplete, IHC)
  3. Chen J, et al. 2009. J. Biol. Chem.. 284:5763 (FC)
Product Citations
  1. Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed
  2. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  3. Li Y, et al. 2022. BMC Pulm Med. 22:180. PubMed
  4. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  5. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  6. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  7. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  8. Yu X, et al. 2016. Blood. 127: 132 - 138. PubMed
  9. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
RRID
AB_10645508 (BioLegend Cat. No. 134319)
AB_10645508 (BioLegend Cat. No. 134320)

Antigen Details

Structure
A transmembrane protein of Ig super family member
Distribution

Expressed on mast and basophils.

Function
Play an important role in triggering IgE-mediated allergic reactions.
Ligand/Receptor
IgE
Cell Type
Basophils, Mast cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105.
2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843.

Gene ID
2205 View all products for this Gene ID
UniProt
View information about FcepsilonRIalpha on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 02/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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