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PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-173 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
400251 25 tests 76€
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400257 25 µg 76€
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400252 100 tests 193€
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400258 100 µg 193€
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Description

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this mouse IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Product Citations
  1. Hu X, et al. 2023. Nat Biotechnol. . PubMed
  2. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  3. Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
  4. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  5. Castro-Gutierrez R, et al. 2021. Front Endocrinol (Lausanne). 12:707881. PubMed
  6. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  7. Stephens M, et al. 2021. Sci Rep. 1.385416667. PubMed
  8. Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
RRID
AB_10695169 (BioLegend Cat. No. 400251)
AB_10695169 (BioLegend Cat. No. 400257)
AB_10695169 (BioLegend Cat. No. 400252)
AB_10695169 (BioLegend Cat. No. 400258)

Antigen Details

Gene ID
NA

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Biotin Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
FITC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PE Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PE/Cyanine5 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Purified Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC,ICC,IHC,IP,WB,FA,ChIP
APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Alexa Fluor® 488 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PE Mouse IgG2a, κ Isotype Ctrl (ICFC) MOPC-173 ICFC
FITC Mouse IgG2a, κ Isotype Ctrl (FC) MOPC-173 FC
PE Mouse IgG2a, κ Isotype Ctrl (FC) MOPC-173 FC
APC Mouse IgG2a, κ Isotype Ctrl (FC) MOPC-173 FC
Brilliant Violet 605™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Alexa Fluor® 700 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PerCP Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Brilliant Violet 570™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 510™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Brilliant Violet 650™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Brilliant Violet 711™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Brilliant Violet 785™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Alexa Fluor® 594 Mouse IgG2a, κ Isotype Ctrl MOPC-173 ICC
FITC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
APC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
TotalSeq™-A0091 Mouse IgG2a, κ isotype Ctrl MOPC-173 PG
TotalSeq™-B0091 Mouse IgG2a, κ isotype Ctrl MOPC-173 PG
TotalSeq™-C0091 Mouse IgG2a, κ isotype Ctrl MOPC-173 PG
PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
KIRAVIA Blue 520™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
Spark Blue™ 550 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Spark NIR™ 685 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Spark Violet™ 538 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Spark YG™ 581 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
TotalSeq™-D0091 Mouse IgG2a, κ Isotype Ctrl MOPC-173 PG
Spark YG™ 593 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP PE Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP FITC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP APC Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE/Fire™ 700 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE/Fire™ 640 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
GMP PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
GMP Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
Spark YG™ 570 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
Spark Violet™ 423 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
APC/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PerCP/Fire™ 806 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PerCP/Fire™ 780 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
Spark Red™ 718 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Spark Blue™ 515 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Spark Blue™ 574 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
PE/Fire™ 744 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC,ICFC
Alexa Fluor® 660 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
Spark UV™ 387 Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC
TotalSeq™-B1472 Mouse IgG2a, κ isotype Ctrl (FC) Antibody MOPC-173 ICPG
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Mouse IgG2a, κ Isotype Ctrl

  • Biotin Mouse IgG2a, κ Isotype Ctrl

  • FITC Mouse IgG2a, κ Isotype Ctrl

  • PE Mouse IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Mouse IgG2a, κ Isotype Ctrl

  • Purified Mouse IgG2a, κ Isotype Ctrl

    MOPC-173_GoChIP_IgG2a_Antibody_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Mouse IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Mouse IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl

  • PE Mouse IgG2a, κ Isotype Ctrl (ICFC)

  • FITC Mouse IgG2a, κ Isotype Ctrl (FC)

  • PE Mouse IgG2a, κ Isotype Ctrl (FC)

  • APC Mouse IgG2a, κ Isotype Ctrl (FC)

  • Brilliant Violet 605™ Mouse IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Mouse IgG2a, κ Isotype Ctrl

  • PerCP Mouse IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Mouse IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Mouse IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Mouse IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Mouse IgG2a, κ Isotype Ctrl

  • FITC Mouse IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl

  • APC Mouse IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl

  • PE Mouse IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0091 Mouse IgG2a, κ isotype Ctrl

  • TotalSeq™-B0091 Mouse IgG2a, κ isotype Ctrl

  • TotalSeq™-C0091 Mouse IgG2a, κ isotype Ctrl

  • PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Mouse IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Mouse IgG2a, κ Isotype Ctrl

  • Spark NIR™ 685 Mouse IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Mouse IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Mouse IgG2a, κ Isotype Ctrl

  • TotalSeq™-D0091 Mouse IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Mouse IgG2a, κ Isotype Ctrl

  • GMP PE Mouse IgG2a, κ Isotype Ctrl

  • GMP FITC Mouse IgG2a, κ Isotype Ctrl

  • GMP APC Mouse IgG2a, κ Isotype Ctrl

  • GMP PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl

  • GMP APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl

  • GMP PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl

  • PE/Fire™ 700 Mouse IgG2a, κ Isotype Ctrl

  • PE/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl

  • PE/Fire™ 640 Mouse IgG2a, κ Isotype Ctrl

  • GMP PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl

  • GMP Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl

  • Spark YG™ 570 Mouse IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Mouse IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 806 Mouse IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 780 Mouse IgG2a, κ Isotype Ctrl

  • Spark Red™ 718 Mouse IgG2a, κ Isotype Ctrl

  • Spark Blue™ 515 Mouse IgG2a, κ Isotype Ctrl

  • Spark Blue™ 574 Mouse IgG2a, κ Isotype Ctrl

  • PE/Fire™ 744 Mouse IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 660 Mouse IgG2a, κ Isotype Ctrl

  • Spark UV™ 387 Mouse IgG2a, κ Isotype Ctrl

  • TotalSeq™-B1472 Mouse IgG2a, κ isotype Ctrl (FC) Antibody

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