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PhosphoPair PLK-1 (Thr210) Antibody Set

Pricing & Availability
Clone
3F8; 2A3;
Regulatory Status
RUO
Other Names
Polo Like Kinase 1,STPK13, Cell Cycle Regulated Protein Kinase
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Product Citations
publications
PhosphoPair_PLK-1_Antibody_Set_081120.png
Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL nocodazole for 20 hours were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PLK-1 Phospho (Thr210) antibody (clone 2A3) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-PLK-1 antibody (clone 3F8) was used as a pan PLK-1 loading control at 1.0 µg/mL. Lane M: Molecular weight marker. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti-PLK-1 antibody (clone 3F8) was used as a pan PLK-1 loading control at 1.0 µg/mL. Lane M: Molecular weight marker.
  • PhosphoPair_PLK-1_Antibody_Set_081120.png
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL nocodazole for 20 hours were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PLK-1 Phospho (Thr210) antibody (clone 2A3) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-PLK-1 antibody (clone 3F8) was used as a pan PLK-1 loading control at 1.0 µg/mL. Lane M: Molecular weight marker. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti-PLK-1 antibody (clone 3F8) was used as a pan PLK-1 loading control at 1.0 µg/mL. Lane M: Molecular weight marker.
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699956 1 kit 151€
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Description

PLK-1 (polo-like kinase 1) is a member of the Polo-like kinase family of serine/threonine protein kinases. While PLK-1 phosphorylates a broad range of targets, it has been shown to be a critical regulator of cell cycle pathways, ranging from mitotic entry to initiation of cytokinesis. Elevated PLK1 expression is described in many cancers and is associated with poor clinical outcomes. Increased PLK-1 activity is associated with resistance to doxorubicin and gemcitabine in certain cancers. Activation of PLK-1 requires phosphorylation of Thr210 within the T-loop of the catalytic domain by Aurora kinases.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-PLK-1 Antibody WB 3F8 25 μg Human, Mouse Mouse IgG1, κ
Purified PLK-1 Phospho (Thr210) Antibody WB 2A3 25 μg Human Mouse IgG1, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation
The antibodies were purified by affinity chromatography.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone 3F8: 0.2 - 1.0 µg/mL
Clone 2A3: 0.5 - 1.0 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Product Citations
  1. Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed

Antigen Details

Structure
PLK1 is a 603 amino acid protein with a predicted molecular weight of ~68 kD.
Distribution

Ubiquitously expressed; highly expressed in placenta and colon/Centrosomes and kinetochore

Function
Mitotic signaling
Antigen References

1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.

Gene ID
5347 View all products for this Gene ID
UniProt
View information about PLK-1 on UniProt.org
Go To Top Version: 1    Revision Date: 08/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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