- Clone
- O93E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PYD and CARD domain-containing protein (PYCARD), Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain protein 5, Target of methylation-induced silencing (TMS1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
676502 | 100 µg | 249€ |
Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1), PYD, and CARD domain containing protein (PYCARD), is a 21.6 kD pro-apoptotic protein that contains a N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm. However, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocates from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Also, the AIM2/ASC complex can act as a novel caspase-8 activation platform. Recent studes has shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits NF-κB activity in primary melanoma. However, in metastatic melanoma through, it has the opposite function enhancing NF-κB activity and the secretion of IL-1β.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length recombinant human PYCARD (NP_037390) produced in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2565643 (BioLegend Cat. No. 676502)
Antigen Details
- Structure
- Full-length ASC (fASC) contains 195 amino acids wtih a predicted molecular weight of 21.6 kD. The sequence of isoform ASC-b is missing 93-111 a.a. The sequence of isoform ASC-c is missing 26-85 a.a.
- Distribution
-
Cytoplasm. In resting monocytes and macrophages, the nucleus is the primary localization.
- Function
- ASC promotes caspase-mediated apoptosis. This pro-apoptotic activity is mediated predominantly through the activation of caspase-9. ASC is an integral component of the inflammasome. Within the inflammasome, ASC links caspase-1 and NOD-like receptors (NLR), which lead to the activation of caspase-1.
- Ligand/Receptor
- CIAS1/PYPAF1 and PYDC1.
- Cell Type
- Dendritic cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
- Antigen References
-
1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
5. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.
7. Liu W, et al. 2013. J. Invest Dermatol. 2:518-527. - Gene ID
- 29108 View all products for this Gene ID
- UniProt
- View information about ASC on UniProt.org
Other Formats
View All ASC Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ASC | O93E9 | WB |
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Purified anti-ASC
Total cell lysate of HL-60 (lane 1, 15 µg), HT-29 (lane 2, 1...
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