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Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# PRB-130P)

Pricing & Availability
Clone
Poly29031 (See other available formats)
Regulatory Status
RUO
Other Names
AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously
Covance Catalog# PRB-130P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Purified_AU1_WB_021315
  • Purified_AU1_WB_021315
Cat # Size Price Quantity Check Availability Save
903101 100 µL 265€
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Description

AU1 is a commonly used epitope that is tagged onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against AU1 epitope recognize proteins containing AU1 epitope tag fused to targeted proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the peptide DTYRYI (the same epitope as the monoclonal AU1 antibody).
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC, ICC, ELISA, Purification
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000-30,000
• ICC: 1:200-2,000
• ELISA: 1:100-400 (coating), 1:1,000-30,000 (primary)
 

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (ICC), immunocytochemistry and ELISA.

This antibody may be preferred over the monoclonal AU1 for experiments in murine systems or in experiments involving two proteins labeled with two different tags. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.


This product may contain other non-IgG subtypes.

Product Citations
  1. Kim GB et al. 2019. Cell. 179(1):251-267 . PubMed
  2. Bodur C, et al. 2018. EMBO J. 37:19. PubMed
RRID
AB_2565028 (BioLegend Cat. No. 903101)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about AU1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All AU1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-AU1 Epitope Tag Poly29031 WB,IHC,ICC,ELISA,Purification
Go To Top Version: 4    Revision Date: 01/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-AU1 Epitope Tag

    Purified_AU1_WB_021315
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