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Purified anti-Bcl11b Antibody

Pricing & Availability
Clone
25B6 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell CLL/lymphoma 11B (zinc finger protein), CTIP2, B-cell lymphoma/leukemia 11B, CTIP-2, hRIT1-alpha, SMARCM2
Isotype
Rat IgG2a
Ave. Rating
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Product Citations
publications
25B6_PURE_Bcl11b_Antibody_1_WB_110317
Total lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2, negative control), and EL4 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Bcl11b Antibody, clone 25B6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-Bcl11b Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 25B6_PURE_Bcl11b_Antibody_1_WB_110317
    Total lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2, negative control), and EL4 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Bcl11b Antibody, clone 25B6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-Bcl11b Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 25B6_PURE_Bcl11b_Antibody_2_IF_083016
    Jurkat cells were stained with anti-bcl11b, clone 25B6 (1:200 dilution), and tubulin-α, clone TU-01, followed by DyLight™ 549 conjugated anti-rat IgG and DyLight™ 488 conjugated anti-mouse IgG. Nuclei are stained with DAPI.
Cat # Size Price Quantity Check Availability Save
650601 25 µg 141€
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650602 100 µg 329€
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Description

The transcription factor Bcl11b is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Zebrafish
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Bcl11b
Formulation
This antibody is provided in Hepes-buffered solution, pH 7.5, 0.02% NaN3.
Preparation
Protein G affinity purified.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB, ICC - Quality tested
CHIP, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and immunofluorescence microscopy. Western blotting, suggested working dilution(s): Use 0.5 µg antibody per ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution of 1:200. The optimal concentration should be determined by titration for each individual assay of interest.

Application References
  1. Tomassy GS, et al. 2010. P. Natl. Acad. Sci. USA 107:3576.
  2. Cherrier T, et al. 2009. Oncogene 28:3380.
  3. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed.
Product Citations
  1. Tian JL, et al. 2023. Cells. 12:. PubMed
  2. Lee SW, et al. 2015. Sci Rep. 16523:5. PubMed
  3. Lo JH, et al. 2018. Mol Cancer Ther. 2377:17. PubMed
  4. Barford K et al. 2017. The Journal of comparative neurology. 525(8):1861-1878 . PubMed
  5. Bott CJ et al. 2019. Mol Biol Cell. 30(10):1214-1229 . PubMed
  6. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed
RRID
AB_10896795 (BioLegend Cat. No. 650601)
AB_10896795 (BioLegend Cat. No. 650602)

Antigen Details

Structure
894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution

Nucleus

Function
Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction
Interacts with TFCOUP1, SIRT1, ARP1, and EAR2
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Liu P, et al. 2011. Immunol. Rev. 238:138.
2. Ikawa T, et al. 2010. Science 329:93.
3. Li L, et al. 2010. Science 329:89.
4. Li P, et al. 2010. Science 329:85.
5. Tydell CC, et al. 2007. J. Immunol. 179:421.
6. Avram D, et al. 2002. Biochem. J. 368:555.

Gene ID
64919 View all products for this Gene ID
UniProt
View information about Bcl11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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