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Purified anti-β-Tubulin Recombinant Antibody

Pricing & Availability
Clone
QA20A98 (See other available formats)
Regulatory Status
RUO
Other Names
β-tubulin, tubulin beta chain (TUBB), tubulin beta-5 chain, TUBB5, TUBB1, CSCSC1, Tubulin Beta class I, Epididymid Secretory Sperm Binding Protein, M40
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A98_PURE_Tubuling_1_090222
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-β-Tubulin recombinant (clone QA20A98) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • QA20A98_PURE_Tubuling_1_090222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-β-Tubulin recombinant (clone QA20A98) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • QA20A98_PURE_Tubuling_2_090222
    IHC staining using purified anti-β-Tubulin recombinant (clone QA20A98) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scale bar = 50 μM
  • QA20A98_PURE_Tubuling_3_090222
    ICC staining of purified anti-β-Tubulin recombinant (clone QA20A98) on A431 cells. The cells were fixed and permeabilized with 4% paraformaldehyde, permeabilized with 0.5% Triton X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective. Scale bar = 50 μM
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607151 25 µg 88€
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607152 100 µg 300€
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Description

Eukaryotic microtubules are tubular polymers of tubulin that are a major component of the cytoskeleton. They play an essential role in diverse cellular functions, including motility, mitosis, and trafficking, and are also critical for normal development. β- and α-tubulins comprise the two core protein families that heterodimerize and assemble into each microtubule filament. There are multiple β-tubulin isotypes, with each showing unique tumoral and tissue-specific distribution. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Amino acids 412-430 of purified bovine brain tubulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, staining was compatible with fixation with 100% ice-cold methanol, or fixation with 4% paraformaldehyde followed by permeabilization with 100% ice-cold methanol or 0.5% Triton-X. 

This antibody is not designed to be specific against any particular isoform of β-tubulin.

RRID
AB_2924621 (BioLegend Cat. No. 607151)
AB_2924621 (BioLegend Cat. No. 607152)

Antigen Details

Structure
β-tubulin is a 444 amino acid protein with a predicted molecular weight of 49.7 kD.
Distribution

β-tubulin is ubiquitously expressed in most cell types

Function
β-tubulin is a GTPase whose polymerization is regulated by GTP hydrolysis
Interaction
β-tubulin homodimerizes with α-tubulin to form microtubules
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References
  1. Lopes D, Maiato H. 2020. Cells. 9:2356. 
  2. Wade RH. 2007. Methods Mol. Med. 137:1-16.
Gene ID
203068 View all products for this Gene ID
UniProt
View information about beta-Tubulin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/02/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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