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Purified anti-Cortactin (CTTN) Antibody

Pricing & Availability
Clone
4F11/CTTN (See other available formats)
Regulatory Status
RUO
Other Names
Amplaxin, Src Substrate Cortactin, Oncogene EMS1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4F11_PURE_Cortactin_Antibody_1_062419.png
Western blot of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F11_PURE_Cortactin_Antibody_1_062419.png
    Western blot of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F11_PURE_Cortactin_Antibody_2_062419.png
    ICC staining of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN) on SH-SY5Y cells. The cells were fixed with 4% PFA, permeabilized with 0.25% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/mL of the primary antibody for 1 hour at room temperature, followed by incubation with 1 µg/ml of Alexa Fluor® 594 conjugated goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. The cells were co-stained with Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) at 1:500 dilution. Nuclei were counterstained with Hoechst 33342, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
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868101 25 µg 85€
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868102 100 µg 221€
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Description

Cortactin is a cytosolic protein that promotes polymerization, rearrangement of the actin cytoskeleton, and synaptogenesis. Stimuli such as alterations in the concentration of growth factors lead to phosphorylation of cortactin by tyrosine or serine/threonine kinases. This modification enables cortactin to bind to filamentous actin and Arp2/3, resulting in the branching of the actin cytoskeleton. In addition, cortactin may be involved in clathrin-independent endocytosis.  Amplification of the cortactin gene is observed in certain tumors, indicating that it may also be a component of tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunocytochemistry, a concentration range of 2 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810812 (BioLegend Cat. No. 868101)
AB_2810812 (BioLegend Cat. No. 868102)

Antigen Details

Structure
Cortactin is a 634 amino acid protein with predicted molecular mass of ~ 71 kD and observed molecular mass of ~ 80 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, and Golgi apparatus

Function
Cortactin promotes polymerization and the rearrangement of the actin cytoskeleton upon external stimulations.
Interaction
SHANK2, SHANK3, FGD1, ABL2, KCNA2, PLXDC2, SRCIN1, SMASN1, ASAP1.
Cell Type
Astrocytes, Endothelial cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Ramos-Garcia P, et al. 2019. Oral Dis. 25(4):949
  2. Schnoor M, et al. 2018. Trends Cell Biol. 28(2):79
  3. Yin M, et al. 2017. Oncotarget. 8(50):88232
Gene ID
2017 View all products for this Gene ID 13043 View all products for this Gene ID 60465 View all products for this Gene ID
UniProt
View information about Cortactin on UniProt.org
Go To Top Version: 1    Revision Date: 06/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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