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Purified anti-Cyclin A Antibody

Pricing & Availability
Clone
E23.1 (See other available formats)
Regulatory Status
RUO
Other Names
CCNA2, cyclin A2, CCN1, CCNA
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_E23point1_PURE_CyclinA_Antibody_WB_031919
Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-Cyclin A Antibody, clone E23.1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of ascending CCNA2 expression (source: Human Protein Atlas).
  • 1_E23point1_PURE_CyclinA_Antibody_WB_031919
    Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-Cyclin A Antibody, clone E23.1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of ascending CCNA2 expression (source: Human Protein Atlas).
  • 2_E23dot1_PURE_Cyclin-A_Antibody_ICFC_1_060617
    NIH/3T3 cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and purified anti-Cyclin A (clone E23.1) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by mouse IgG PE.
  • 3_E23dot1_PURE_Cyclin-A_Antibody_ICFC_2_060617
  • E23dot1_PURE_CyclinA_Antibody_4_012022
    Jurkat cells were fixed and permeabilized with cold 70% ethanol then intracellularly stained with DAPI and purified anti-Cyclin A (clone E23.1) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by mouse IgG PE.
  • E23dot1_PURE_CyclinA_Antibody_5_012022
Cat # Size Price Quantity Check Availability Save
644001 25 µg 90€
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Description

Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cyclin A fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg per ml antibody dilution buffer. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Danno S, et al. 2017. Genes Cells. 22:220. PubMed
RRID
AB_2071969 (BioLegend Cat. No. 644001)

Antigen Details

Distribution

55 kd protein

Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Pagano M et al. 1992. EMBO J 11:961
2. Geley S et al. 2001 J Cell Biol. 153:137
3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347
4. Furuno N et al. 1999. J Cell Biol. 147:295

Gene ID
890 View all products for this Gene ID
UniProt
View information about Cyclin A on UniProt.org
Go To Top Version: 5    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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