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Purified anti-DbH Antibody

Pricing & Availability
Clone
Poly28585 (See other available formats)
Regulatory Status
RUO
Other Names
DBH, Dopamine Beta-Hydroxylase
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28585_Pure_DbH_Antibody_1_040519
Western blot of purified anti-DbH antibody (clone Poly28585). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of HEK293 cell lysate; Lane 4: 20ug of mouse heart lysate (negative control). The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28585_Pure_DbH_Antibody_1_040519
    Western blot of purified anti-DbH antibody (clone Poly28585). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of HEK293 cell lysate; Lane 4: 20ug of mouse heart lysate (negative control). The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28585_Pure_DbH_Antibody_2_040519
    IHC staining of purified anti-DbH antibody (clone Poly28585) on formalin-fixed paraffin-embedded human adrenal gland tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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858501 25 µg 100€
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Description

Dopamine beta-hydroxylase (DbH) is a homotetramer enzyme that catalyzes the chemical reaction that converts dopamine to norepinephrine. It is the only known membrane-bound enzyme capable of synthesizing a small-molecule inside synaptic vesicles. DbH is expressed in noradrenergic nerve terminals and in chromaffin cells of the adrenal medulla. Clinically, low levels of DbH expression is identified as DbH deficiency, and has been shown to correlate with conditions associated with addiction, decision making, ADHD and Alzheimer’s Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant DbH (His335~Val571) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801220 (BioLegend Cat. No. 858501)

Antigen Details

Structure
DbH is a 617 amino acid protein with an apparent molecular mass of ~ 69 kD.
Distribution

Tissue Distribution: Adrenal gland, colon, and brain
Cellular Distribution: Extracellular, cytoskeleton, and endoplasmic reticulum

Function
DbH is the enzyme that catalyzes the chemical reaction to convert dopamine to norepinephrine.
Interaction
Forms homotetramer
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Enzymes and Regulators, Presynaptic proteins
Antigen References
  1. Nagatsu T, et al. 2016. J Neural Transm (Vienna). 123(11):1255
  2. Patriquin MA, et al. 2015. Psychiatr Genet. 25(5):181
  3. Kopeckova M, et al. 2006. Neuro Endocrinol Lett. 27(6):748
Gene ID
1621 View all products for this Gene ID
UniProt
View information about DbH on UniProt.org
Go To Top Version: 1    Revision Date: 04/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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