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Purified anti-DLAT Antibody

Pricing & Availability
Clone
4A4-B6-C10 (See other available formats)
Regulatory Status
RUO
Other Names
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT), dihydrolipoyl transacetylase (DLTA), Pyruvate dehydrogenase complex component E2 (PDC-E2; PDCE2), Dihydrolipoamide S-Acetyltransferase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. 4A4-B6-C10_PURE_DLAT_WB_041823
Whole cell extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-DLAT (clone 4A4-B6-C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. 4A4-B6-C10_PURE_DLAT_WB_041823
    Whole cell extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-DLAT (clone 4A4-B6-C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. 4A4-B6-C10_PURE_DLAT_ICC_1_041823
    HeLa cells were fixed with 4% Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 2.5 µg/mL of purified mouse IgG1, κ isotype control (Cat. No. 401402) (panel A) or purified anti-DLAT (clone 4A4-B6-C10) (panel B), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • c. 4A4-B6-C10_PURE_DLAT_ICC_2_041823
    HeLa cells were fixed with 4% Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 2.5 µg/mL of purified anti-DLAT (clone 4A4-B6-C10), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) and MitoSpy™ Red CMXRos (Cat. No. 424801). Panel A: DLAT + DAPI, Panel B: DAPI + MitoSpy™, Panel C: DLAT + DAPI + MitoSpy™. Images were captured with a 60X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
614251 25 µg 116€
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614252 100 µg 332€
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Description

Dihydrolipoamide S-Acetyltransferase (DLAT) is one of three enzyme components of the pyruvate dehydrogenase complex. Specifically, DLAT catalyzes the transfer of an acetyl group to coenzyme A (CoA), which is the second step in the conversion of pyruvate into acetyl CoA during the tricarboxylic acid (TCA) cycle. 

Autoimmune reactivity against DLAT is the most common cause of primary biliary cholangitis, an autoimmune disease caused by autoantibodies against mitochondrial antigens. Additionally, mutations in this gene can cause pyruvate dehydrogenase E2 deficiency, resulting in primary lactic acidosis in infancy and early childhood. In line with the Warburg Hypothesis, which posits that disrupted cellular metabolism drives cancer formation, upregulation of DLAT is a characteristic of some cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human DLAT fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in IHC-P. 

This product does not recognize mouse DLAT in WB. 

When testing by ICC, the following fixation and permeabilization methods are suitable: ice-cold methanol, 4% PFA + 0.5% Triton-X, and 4%PFA + 100% methanol.

RRID
AB_2941616 (BioLegend Cat. No. 614251)
AB_2941616 (BioLegend Cat. No. 614252)

Antigen Details

Structure
DLAT is a 647 amino acid protein with a predicted molecular weight of 69.0 kD
Distribution

Ubiquitous/ mitochondria

Function
DLAT catalyzes the acetylation of coenzyme A
Interaction
DLAT is the E2 component of the pyruvate dehydrogenase complex along with pyruvate dehydrogenase (E1), dihydrolipoyl dehydrogenase (E3), and dihydrolipoyl dehydrogenase binding protein (E3BP)
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators, Mitochondrial Markers
Antigen References
  1. Mackay IR, et al. 2000. Immunological Rev. 174:226-37. 
  2. Head RA, et al. 2005. Ann Neurol. 58:234-41. 
  3. Goh WQ, et al. 2015. Am J Transl Res. 7:1140-51.

 

Gene ID
1737 View all products for this Gene ID
UniProt
View information about DLAT on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/18/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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