- Clone
- P83G4B6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNM1, DNM, DMN-1, dynamin 1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P83G4B6_PURE_Dynamin1_Antibody_1_091417 P83G4B6_PURE_Dynamin1_Antibody_1_091417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P83G4B6_PURE_Dynamin1_Antibody_1_HR_091417.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder. -
Total lysates (15 µg protein) from human brain, mouse brain and rat brain were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody. 1:1000 (0.5 micro;g/mL) dilution of β-actin (2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder. -
IHC staining of Formalin Fixed Paraffin Embedded (FFPE) normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. -
IHC staining of Formalin Fixed Paraffin Embedded (FFPE) mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. -
IHC staining of Formalin Fixed Paraffin Embedded (FFPE) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
604001 | 25 µg | 81€ | ||||
604002 | 100 µg | 203€ |
Dynamin-1, also known as DMN-1, is a 100 kD protein with GTPase activity. Dynamin-1 is involved in several intracellular trafficking processes including synaptic vesicle recycling, neurotransmitter reuptake and receptor internalization. In addition, Dynamin-1 may be necessary for the establishment and proper maintenance of mature neuronal structure. Studies have shown that Dynamin-1 is upregulated during new neurite formation. Conversely, Dynamin-1 is downregulated during neurite retraction. Silencing the initiation codon for Dynamin limits the formation of axon-like structures.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Dynamin-1 (515-750a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 – 2 µg/mL). For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1:100-1:250 diluted (2 – 5 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2716194 (BioLegend Cat. No. 604001)
AB_2716194 (BioLegend Cat. No. 604002)
Antigen Details
- Structure
- 864 amino acids with a predicted molecular weight of 97 kD.
- Distribution
-
Cytoplasm
- Function
- Involved in producing microtubule bundles and able to bind and hydrolyze GTP
- Interaction
- Interacts with CAV1, SH3GLB1, SH3GL1, SH3GL2, SH3GL3, PHOCN, PACSIN1, PACSIN2, PACSIN3, SNX9, MYO1E, SNX33, UNC119 and DIAPH1.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Obar RA, et al. 1990. Nature. 347:256.
- Chen-Hwang MC, et al. 2002. J Biol Chem. 277:17597.
- Grabs D, et al. 1997. J Biol Chem. 272:13419.
- Wunderlich L, et al. 1999. Cell. Signal. 11:25.
- Gene ID
- 1759 View all products for this Gene ID
- UniProt
- View information about Dynamin-1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Dynamin-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Dynamin-1 | P83G4B6 | WB,IHC-P |
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Purified anti-Dynamin-1
Total lysates (15 µg protein) from Raji (negative control), ... Total lysates (15 µg protein) from human brain, mouse brain ... IHC staining of Formalin Fixed Paraffin Embedded (FFPE) norm... IHC staining of Formalin Fixed Paraffin Embedded (FFPE) mous... IHC staining of Formalin Fixed Paraffin Embedded (FFPE) rat ...
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