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Purified anti-FAP Antibody

Pricing & Availability
Clone
A21107A (See other available formats)
Regulatory Status
RUO
Other Names
Prolyl endopeptidase FAP, fibroblast activation protein alpha (FAP-alpha), 170 kDa melanoma membrane-bound gelatinase, dipeptidyl peptidase FAP (DPPIV), Integral membrane serine protease, Post-Proline Cleaving Enzyme, SIMP
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A21107A_PURE_FAP_WB_081123
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-FAP (clone A21107A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A21107A_PURE_FAP_WB_081123
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-FAP (clone A21107A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A21107A_PURE_FAP_IHCP_081123
    IHC staining of purified anti-FAP (clone A21107A) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated with 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) (panels A and B) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A). Images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
621151 25 µg 140€
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621152 100 µg 360€
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Description

Fibroblast activation protein (FAP) is a cell surface and soluble protein with diverse functions in tissue homeostasis and diseases. As a prolyl peptidase, FAP cleaves peptide bonds following a proline residue. Primarily expressed by activated fibroblasts, FAP plays a crucial role in tissue remodeling and repair. Capable of being shed into the cellular milieu, FAP is involved in the breakdown and modification of the extracellular matrix, which is essential for tissue regeneration during wound healing and tissue repair. Moreover, FAP has been found to be overexpressed in the tumor microenvironment, where it potentially promotes tumor growth, invasion, and metastasis. This association with cancer has led to investigations exploring FAP as a potential therapeutic target for cancer treatment. FAP is inactive when monomeric and must form a homodimer to become functional for both the cell surface and soluble forms. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human FAP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Product is not suitable for ICC.

Product does not react with mouse FAP in Western Blot.

For IHC-P, antigen retrieval using sodium citrate, pH 6.0 is recommended. Tris-EDTA-based antigen retrieval solutions are not recommended.

RRID
AB_3083407 (BioLegend Cat. No. 621151)
AB_3083407 (BioLegend Cat. No. 621152)

Antigen Details

Structure
FAP is a 760 amino acid protein with a predicted molecular weight of 87.7 kD.
Distribution

Cell surface, secreted

Function
Protease
Interaction
Degrades gelatin, DPP4, integrin alpha-3/beta-1, ITGB1, PLAUR
Cell Type
Fibroblasts
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Proteases
Antigen References
  1. Lee KN, et al. 2006. Blood. 107:1397-404.
  2. Waumans Y. et al. 2015. Front Immunol. 6:387.
  3. Kotackova L, et al. 2009. Folia Biol. (Praha). 55:77-84.
  4. Sulda ML, et al. 2006. Adv Exp Med Biol. 575:197.
  5. Aertgeerts K, et al. 2005. J Biol Chem. 280:19441-4.
  6. Mueller S, et al. 1999. J Biol Chem. 274:24947-52.
Gene ID
2191 View all products for this Gene ID
UniProt
View information about FAP on UniProt.org
Go To Top Version: 1    Revision Date: 08/11/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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