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Purified anti-Hippocalcin Antibody

Pricing & Availability
Clone
A18228E (See other available formats)
Regulatory Status
RUO
Other Names
HPCA, Neuron-specific calcium-binding protein hippocalcin, Calcium-Binding Protein BDR-2, BDR-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18228E_PURE_Hippocalcin_WB_1_061623
Whole cell extracts (15 µg total protein) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-hippocalcin (clone A18228E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A18228E_PURE_Hippocalcin_WB_1_061623
    Whole cell extracts (15 µg total protein) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-hippocalcin (clone A18228E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A18228E_PURE_Hippocalcin_WB_2_061623
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-hippocalcin (clone A18228E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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619251 25 µg 133€
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619252 100 µg 332€
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Description

Hippocalcin, a member of the neuronal calcium (Ca2+) sensor family, is a Ca2+-binding protein that translocates between membrane and cytosol through its Ca2+/myristoyl switch. Its structural composition consists of 191 amino acids, forming a compact protein with a molecular weight of approximately 21 kD. The protein is characterized by the presence of four EF-hand motifs, each containing a helix-loop-helix structure, facilitating high-affinity Ca2+ binding. Through its Ca2+-sensing capabilities, hippocalcin modulates various crucial cellular functions and signaling pathways. It participates in the regulation of intracellular Ca2+ homeostasis and influences Ca2+-dependent signaling cascades. The protein's interactions with key proteins and enzymes enables it to contribute to processes such as neurotransmitter release, synaptic plasticity, and neuronal excitability. Hippocalcin’s involvement in human diseases has been observed in several conditions. In neurological disorders like epilepsy, dysregulation of hippocalcin disrupts Ca2+ signaling, leading to neuronal hyperexcitability and the manifestation of seizures. Additionally, alterations in hippocalcin expression and function have been linked to neurodegenerative diseases, including Alzheimer's and Parkinson's, suggesting its potential role in disease progression. Furthermore, elevated levels of hippocalcin have been associated with certain cancers, such as breast and lung cancer, indicating its involvement in tumor progression and metastasis.
Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of Hippocalcin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not suitable for immunocytochemistry (ICC) and immunohistochemistry (IHC-P). 

RRID
AB_3068101 (BioLegend Cat. No. 619251)
AB_3068101 (BioLegend Cat. No. 619252)

Antigen Details

Structure
Ca2+ -dependent oligomerization
Distribution

Abundant in brain and hippocampus; membrane/cytoplasmic translocation

Function
Bind to Ca2+ to regulate neuronal activity
Interaction
β2-adaptin subunit of the AP2 adaptor complex, PSD95
Ligand/Receptor
Ca2+
Cell Type
Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Neuroscience, Signal Transduction
Molecular Family
Postsynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. O'Callaghan DW, et al. 2003. J Cell Biol. 163:715-21.
  2. Palmer CL, et al. 2005. Neuron. 47:487-94.
  3. Kobayashi M, et al. 2005. Neuroscience. 133:471-84.
  4. Kerrigan TL, et al. 2017. Front Mol Neurosci. 5:57.
  5. Park S-Y, et al. 2017. Stem Cell Reports. 8:95-111.
Gene ID
3208 View all products for this Gene ID
UniProt
View information about Hippocalcin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/16/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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