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Purified anti-Histone H3 Dimethyl (Lys9) Antibody (Previously Covance catalog# MMS-5078)

Pricing & Availability
Clone
5E5-G5 (See other available formats)
Regulatory Status
RUO
Other Names
Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously
Covance Catalog# MMS-5078
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_1_090518
Western blot of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear fraction lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_1_090518
    Western blot of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear fraction lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_2_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_3_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_4_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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815501 100 µL 268€
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Description

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, di-methylated at Lysine-9.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
RRID
AB_2564793 (BioLegend Cat. No. 815501)

Antigen Details

Structure
Expected MW: 17 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction
Antigen References
  1. Murzina N, et al. 2008. Structure. 16:1077.
  2. Chen S, et al. 2015. Genes & Dev. 29:1326.
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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