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Purified anti-human CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
H4B4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LAMPb, LGP110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_H4B4_FITC_CD107b_Antibody_1_FC_011316
Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with CD107b (clone H4B4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • A_H4B4_FITC_CD107b_Antibody_1_FC_011316
    Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with CD107b (clone H4B4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • B_H4B4_PURE_CD107b_Antibody_2_IHCP_022819
    Human paraffin-embedded prostate tissue slice was stained with purified anti-human CD107b (clone H4B4) followed by goat anti-mouse IgG (clone Poly4053) DyLight™ 488 (green) and anti-human CD44 (clone IM7) Alexa Fluor® 594 (red). The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. See additional supplemental data for detailed information.
  • C_H4B4_PURE_CD107a_LAMP1_ICC_022718
    ICC staining of purified anti-LAMP-2 antibody (clone H4B4) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.
  • D_H4B4_PURE_CD107a_LAMP1_WB_022718
    Western blot of purified anti-LAMP-2 antibody (clone H4B4). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysates; Lane 5: 20 µg of mouse brain lysates; Lane 6: 10 ng human recombinant LAMP-2. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Anti-β-actin antibody was used as the loading control (Cat. No. 643807). Enhanced chemiluminescence was used as the detection system.
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354301 25 µg 48€
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354302 100 µg 92€
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Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Adult human adherent spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IHC-P, ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of  neutrophils2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References
  1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
  2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
  3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
Product Citations
  1. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  2. de Leeuw SM, et al. 2022. Stem Cell Reports. 17:110. PubMed
  3. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  4. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  5. Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
RRID
AB_11204081 (BioLegend Cat. No. 354301)
AB_11204081 (BioLegend Cat. No. 354302)

Antigen Details

Structure
LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution

Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines

Function
Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type
B cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538.

Gene ID
3920 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org
Go To Top Version: 3    Revision Date: 02/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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