- Clone
- MHR73-11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD180, Radio-protective 105-kD protein, RP105
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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312902 | 100 µg | 156€ |
CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human RP105 complexed with mouse MD-1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICFC, IHC-F, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: intracellular staining1, immunoprecipitation1, immunohistochemistry1 of acetone-fixed frozen tissue sections, B cell activation1, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).
- Application References
-
- Miura Y, et al. 1998. Blood 92:2815.
- Indrevaer RL, et al. 2013. J. Immunol. 191:3624. PubMed
- Product Citations
-
- RRID
-
AB_2228772 (BioLegend Cat. No. 312902)
Antigen Details
- Structure
- Member of the toll-like receptor family, 105 kD
- Distribution
-
B cells, monocytes/macrophages, and dendritic cells
- Function
- Signaling protein involved in innate immunity
- Ligand/Receptor
- LPS
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Miura Y, et al. 1998. Blood 92:2815.
- Gene ID
- 4064 View all products for this Gene ID
- UniProt
- View information about CD180 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD180 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD180 (RP105) | MHR73-11 | FC,ICFC,IHC-F,IP |
PE anti-human CD180 (RP105) | MHR73-11 | FC |
PE/Cyanine7 anti-human CD180 (RP105) | MHR73-11 | FC |
APC anti-human CD180 (RP105) | MHR73-11 | FC |
Ultra-LEAF™ Purified anti-human CD180 (RP105) | MHR73-11 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with purified MHR... -
PE anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with MHR73-11 PE -
PE/Cyanine7 anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with CD180 (clone... -
APC anti-human CD180 (RP105)
Human peripheral blood lymphocytes were stained with CD180 (... -
Ultra-LEAF™ Purified anti-human CD180 (RP105)
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ...
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