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Purified anti-ICAM-2 Antibody

Pricing & Availability
Clone
Poly5440 (See other available formats)
Regulatory Status
RUO
Other Names
Intracellular adhesion molecule 2 (ICAM2), Cluster of Differentiation 102 (CD102)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5440_PURE_ICAM2_Antibody_041023
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-ICAM-2 (clone Poly5440) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly5440_PURE_ICAM2_Antibody_041023
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-ICAM-2 (clone Poly5440) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly5440_PURE_ICAM2_Antibody_2_041023
    Whole cell extracts (15 µg total protein) from indicated cells spiked with 20 ng of recombinant human ICAM-1 (Cat. No. 552904) and recombinant human ICAM-2 (Cat. No. 768506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-ICAM-2 (clone Poly5440) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat secondary antibody. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Please note that this recombinant ICAM-2 migrates at approximately 75 kDa and 150 kDa depending on reducing conditions. Lane M: Molecular weight marker
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544001 50 µL 236€
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Description

Intracellular adhesion molecule 2 (ICAM2) is a type I transmembrane glycoprotein that mediates cell-cell adhesions. Specifically, ICAM2 binds to LFA-1, a member of the integrin family primarily expressed by lymphocytes. ICAM2 expression regulates the migration of leukocytes and neutrophils via cell-cell interactions and plays an important role in their development and immune response. Additionally, ICAM2 controls the adhesion of developing sperm in the testis and is important for proper spermatogenesis. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant fragment of human ICAM-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not suitable for IHC-P. 

This product does not cross-react with human ICAM-1 in Western Blot. 

RRID
AB_2941599 (BioLegend Cat. No. 544001)

Antigen Details

Structure
ICAM-2 is a 275 amino acid protein with an estimated molecular weight of 30.6 kD.
Distribution

Plasma membrane, cytosol

Function
Cell-cell adhesion
Ligand/Receptor
Lymphocyte function-associated antigen 1 (LFA-1)
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Xiao X, et al. 2013. Hum Reprod Update. 19:167-86.
  2. Lyck R, et al. 2015. Curr Opin Hematol. 22:53-9.
  3. Gahmberg CG, et al. 1998. Cell Mol Life Sci. 54:549-55.
Gene ID
3384 View all products for this Gene ID
UniProt
View information about ICAM-2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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