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Purified anti-human/mouse CD266 (Fn14, TWEAK R) Antibody

Pricing & Availability
Clone
ITEM-4 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast growth factor-inducible 14 kD protein (Fn14), TWEAK receptor, TNFRSF12A
Isotype
Mouse IgG2b, κ
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Product Citations
publications
ITEM-4_Purified_090707
Human Fn14 transfected cells stained with purified ITEM-4, followed by anti-mouse IgG FITC
  • ITEM-4_Purified_090707
    Human Fn14 transfected cells stained with purified ITEM-4, followed by anti-mouse IgG FITC
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314102 100 µg 184€
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Description

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Fn14 transfected P815
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-P, IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting3,4, immunofluorescence3,4, and immunohistochemistry of frozen sections5 and formalin-fixed paraffin-embedded sections3. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells1.

Application References
  1. Polek TC, et al. 2003. J. Biol. Chem. 278:32317.
  2. Nakayama M, et al. 2003. J. Immunol. 170:341.
  3. Karaca G, et al. 2014. PLoS One. 9:83987. (IHC, IF, WB) PubMed
  4. Muñoz-García B, et al. 2006. Stroke. 37:2044. (IF, WB)
  5. Jakubowski A, et al. 2005. J. Clin. Invest. 115:2955. (IHC)
  6. Karaca G, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308:325. PubMed
Product Citations
  1. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  2. Chen Y, et al. 2011. PLoS One. 6:e22202. PubMed
  3. Karaca G, et al. 2014. PLoS One. 9:83987. PubMed
  4. Karaca G, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308:325. PubMed
  5. Salahudeen AA, et al. 2020. Nature. 588:670. PubMed
RRID
AB_2240752 (BioLegend Cat. No. 314102)

Antigen Details

Structure
Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution

Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC

Function
Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand/Receptor
TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.

Gene ID
27279 View all products for this Gene ID 51330 View all products for this Gene ID
UniProt
View information about CD266 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/14/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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